Vaccination of pigs with a recombinant porcine adenovirus expressing the gD gene from pseudorabies virus
Vaccination of pigs with a recombinant porcine adenovirus expressing the gD gene from pseudorabies virus
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DOI：
10.1016/s0264-410x(01)00084-6
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发表时间：
2001-06-14
期刊：
影响因子：
5.5
通讯作者：
Johnson, MA
中科院分区：
文献类型：
--
作者：
Hammond, JM;Jansen, ES;Johnson, MA
用单剂量或两剂量的表达来自伪狂犬病病毒（PRV）的糖蛋白D基因的重组猪腺病毒接种5周龄的市售大白色猪。监测猪的PRV血清中和抗体的产生，并在最终接种后3周进行攻毒。攻毒前，接种2剂疫苗的猪与接种单剂疫苗的猪相比，抗体水平升高，所有存活猪的中和滴度均高于攻毒前水平。攻毒后，监测猪的疾病临床体征，采集血液和鼻拭子进行病毒分离。所有对照动物在攻毒后6天内因体温升高而患病，而接种疫苗的动物仅在2-3天内体温升高。对照组和单次给药组的猪都失去了状态。但给予2剂的组保持健康。尸检时，仅在对照组动物和接种单剂量疫苗的动物中发生肺炎的肉眼病变。对所有动物的脑进行的组织学检查表明，试验动物之间的感染严重程度和免疫组织化学抗原检测频率存在差异。对照组和单次给药组受影响最严重，2次给药组受影响最少。病毒分离研究表明，未检出病毒血症，但攻毒后，在两组疫苗接种组中一些动物的鼻拭子中均检出病毒。(C)2001爱思唯尔科技有限公司版权所有。
Five week old, commercially available large white pigs were vaccinated with either a single dose or two doses of a recombinant porcine adenovirus expressing the glycoprotein D gene from pseudorabies virus (PRV). Pigs were monitored for the development of serum neutralizing antibodies to PRV and challenged 3 weeks after final vaccination. Prior to challenge, pigs given 2 doses of the vaccine demonstrated boosted levels of antibody compared with those given a single dose, and all surviving pigs had increased neutralization titres over pre-challenge levels. Following challenge, pigs were monitored for clinical signs of disease, with blood and nasal swabs collected for virus isolation. All control animals became sick with elevated temperatures for 6 days post challenge, whereas; vaccinated animals displayed an increase in body temperature for only 2-3 days. Control pigs and those given a single dose all lost condition. but the group given 2 doses remained healthy. At postmortem, gross lesions of pneumonia only occurred in control animals and those given a single dose of vaccine. Histology carried out on the brains of all animals demonstrated a difference in severity of infection and frequency of immunohistochemical antigen detection between test animals. with control and single dose groups being most severely affected and pigs given 2 doses the least. Virus isolation studies demonstrated that no viraemia could be detected, but virus was found in nasal swabs from some animals in both groups of vaccinates following challenge. (C) 2001 Elsevier Science Ltd. All rights reserved.