Isolation, characterization, and quantitative analysis of C-reactive protein from horses.
Isolation, characterization, and quantitative analysis of C-reactive protein from horses.
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马 C 反应蛋白的分离、表征和定量分析。
DOI：
10.2460/ajvr.1990.51.08.1215
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发表时间：
1990
影响因子：
1
通讯作者：
Kanjuro Otomo
中科院分区：
文献类型：
--
作者：
Mitsuyoshi Takiguchi;Toru Fujinaga;Masaharu Naiki;Shinya Mizuno;Kanjuro Otomo
采用钙依赖性亲和层析结合肺炎球菌C-多糖、阴离子交换层析和凝胶过滤从马血清中分离C-反应蛋白（CRP）。通过其与抗人CRP的免疫化学交叉反应性、其与人CRP的氨基酸组成同源性以及电子显微镜显示的五聚体结构，将其鉴定为真正的CRP。纯化的马CRP分子量约为118，000，由5个相同的非糖基化和非共价缔合亚基组成，每个亚基的分子量约为23，000。免疫电泳结果表明，马CRP迁移于β-球蛋白和γ-球蛋白之间，其等电点约为7.0。在马中，CRP浓度升高与临床肺炎、肠炎和关节炎相关，与使用单向免疫扩散法在临床正常马中获得的值相比。IM给予松节油或去势后，血清CRP浓度升高至基线值的6倍。结果表明，CRP可能是一种急性时相反应蛋白在马。
C-reactive protein (CRP) was isolated from equine serum by use of calcium-dependent affinity chromatography conjugated pneumococcal C-polysaccharide, anion exchange chromatography, and gel filtration. It was identified as genuine CRP by its immunochemical cross-reactivity with anti-human CRP, its homology with human CRP in amino acid composition, and its pentameric structure as revealed by electron microscopy. Purified equine CRP had a molecular weight of approximately 118,000 and was composed of 5 identical, nonglycosylated and noncovalently associated subunits with molecular weight of approximately 23,000 each. Equine CRP migrated in the region between beta- and gamma-globulin by results of immunoelectrophoresis, and its isoelectric point was about 7.0. In horses, increased CRP concentration was associated with clinical pneumonitis, enteritis, and arthritis, compared with values obtained in clinically normal horses by use of single radial immunodiffusion method. After IM administration of turpentine oil or castration, serum CRP concentration increased to 6 times higher than baseline values. Results indicate that CRP may be an acute-phase reactant protein in horses.