Stable incorporation of a bacterial gene into adult rat skeletal muscle in vivo.
Stable incorporation of a bacterial gene into adult rat skeletal muscle in vivo.
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细菌基因在体内稳定掺入成年大鼠骨骼肌中。
DOI：
10.1152/ajpcell.1990.258.3.c578
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Booth,FW
中科院分区：
文献类型：
--
作者：
Thomason,DB;Booth,FW
我们已经开发了一种新的技术，将外源基因在成年大鼠骨骼肌在体内稳定表达。用注射的逆转录病毒感染布比卡因损伤再生的骨骼肌中的内源性卫星细胞，所述逆转录病毒含有在莫洛尼鼠白血病病毒长末端重复序列的启动子控制下的大肠杆菌β-半乳糖苷酶基因。肌肉再生6天和1个月后，在肌纤维中观察到组成性和稳定的β-半乳糖苷酶活性表达。观察到两种表达模式，纤维内弥漫性表达和与肌膜相关的局灶性表达。这项技术将允许未来的实验与肌肉特异性基因和启动子，以研究在完整的成年哺乳动物骨骼肌基因表达的生理调节。此外，刺激干细胞增殖以允许逆转录病毒介导的基因转移的技术通常可适用于其他组织。
We have developed a novel technique to incorporate and stably express foreign genes in adult rat skeletal muscle in vivo. Endogeneous satellite cells in skeletal muscle regenerating from bupivacaine damage were infected with an injected retrovirus containing the Escherichia coli beta-galactosidase gene under the promoter control of the Moloney murine leukemia virus long-terminal repeat. Constitutive and stable expression of beta-galactosidase activity was observed in muscle fibers after 6 days and 1 mo of muscle regeneration. Two patterns of expression were observed, diffuse expression within fibers and focal expression associated with the sarcolemma. This technique will allow future experiments with muscle-specific genes and promoters to study the physiological regulation of skeletal muscle gene expression in the intact adult mammal. Furthermore, the technique of stimulating stem cell proliferation to allow retroviral-mediated gene transfer may be generally applicable to other tissues.