Invasiveness of Salmonella serotypes Typhimurium, Choleraesuis and Dublin for rabbit terminal ileum in vitro
Invasiveness of Salmonella serotypes Typhimurium, Choleraesuis and Dublin for rabbit terminal ileum in vitro
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DOI：
10.1099/00222615-48-9-801
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发表时间：
1999-09-01
影响因子：
3
通讯作者：
Stephen, J
中科院分区：
文献类型：
--
作者：
Bolton, AJ;Martin, GD;Stephen, J
10个最近的临床分离的沙门氏菌血清型鼠伤寒沙门氏菌从人进行了测试，他们的侵入性在兔回肠外植体在体外，与鼠伤寒菌株TML，一个良好的特点，从人类胃肠炎的情况下分离的侵入性菌株进行了比较。10株中有9株的侵袭力与TML相当，1株（GM 3）的侵袭力明显低于TML;电子显微镜显示该菌株具有组织毒性-这可能是其从回肠粘膜恢复减少并因此具有明显的“低”侵袭性的原因，猪霍乱沙门氏菌血清型菌株A50，分离自猪的系统性沙门氏菌病病例，和血清型都柏林菌株3246，分离自小牛系统性沙门氏菌病的情况下，也进行了检查。都柏林菌株3246在37 ℃下生长并立即用于侵袭测定时，以与鼠伤寒沙门氏菌菌株GM 3相似的方式破坏粘膜，而都柏林菌株3246在37 ℃下生长并在4 ℃下储存过夜则没有。这反映在与储存在4 ℃下的生物体相比，新鲜生长的生物体的入侵性明显较低。相反，鼠伤寒沙门氏菌GM 3菌株的组织毒性不受4 ℃储存的影响。当在4 ℃下储存时，猪霍乱菌株A50和都柏林菌株3246的侵袭性水平彼此之间或与鼠伤寒菌株TML没有显著差异。
Ten recent clinical isolates of Salmonella serotype Typhimurium from man that were tested for their invasiveness in rabbit ileal explants in vitro, were compared with Typhimurium strain TML, a well-characterised invasive strain isolated from a case of human gastro-enteritis. Nine of the 10 strains showed invasiveness that was comparable to that of strain TML, One isolate (GM3) was apparently substantially less invasive; electron microscopy showed this strain to be histotoxic - the probable reason for its reduced recovery from ileal mucosa and thus apparent 'low' invasiveness, Salmonella serotype Choleraesuis strain A50, isolated from a case of systemic salmonellosis in pigs, and serotype Dublin strain 3246, isolated from a case of systemic salmonellosis in calves, were also examined. Dublin strain 3246, when grown at 37 degrees C and used immediately in the invasion assay, damaged the mucosa in a manner similar to that of Typhimurium strain GM3, whereas Dublin strain 3246 grown at 37 degrees C and stored overnight at 4 degrees C did not. This was reflected in an apparently lower invasiveness of freshly grown organisms compared with that of organisms stored at 4 degrees C. In contrast, the histotoxicity of Typhimurium strain GM3 was not affected by storage at 4 degrees C. When stored at 4 degrees C, the levels of invasiveness of Choleraesuis strain A50 and Dublin strain 3246 were not significantly different from each other or from Typhimurium strain TML.