Chemical modification as an approach to elucidation of sodium pump structure-function relations.
Chemical modification as an approach to elucidation of sodium pump structure-function relations.
复制标题
化学修饰作为阐明钠泵结构-功能关系的一种方法。
DOI：
10.1152/ajpcell.1990.258.1.c1
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kaplan,JH
中科院分区：
文献类型：
--
作者：
Pedemonte,CH;Kaplan,JH
对酶中特定残基进行化学修饰，并表征其抑制类型和修饰活性的性质，是蛋白质结构-功能研究中的一种既定方法。近年来，随着从基因克隆技术中获得初级序列信息的进展，这种策略变得更加富有成效。本文讨论了化学修饰方法在Na（+）-K（+）-ATP酶研究中的应用。由于抑制剂、配体、修饰剂和蛋白水解酶的联合使用，关于动力学、酶结构和各种构象状态的大量信息已经变得可用。我们将回顾各种试剂和方法，已被用来达到结构-功能相关性，并严格讨论从这些方法获得的信息类型的限制和模糊性。Na（+）泵蛋白的化学修饰已经提供了大量的数据，我们预计，当合适的表达系统变得可用时，将指导未来诱变研究的努力。
Chemical modification of specific residues in enzymes, with the characterization of the type of inhibition and properties of the modified activity, is an established approach in structure-function studies of proteins. This strategy has become more productive in recent years with the advances made in obtaining primary sequence information from gene-cloning technologies. This article discusses the application of chemical modification procedures to the study of the Na(+)-K(+)-ATPase protein. A wide array of information has become available about the kinetics, enzyme structure, and various conformational states as a result of the combined use of inhibitors, ligands, modifiers, and proteolytic enzymes. We will review a variety of reagents and approaches that have been employed to arrive at structure-function correlates and discuss critically the limits and ambiguities in the type of information obtained from these methodologies. Chemical modification of the Na(+)-pump protein has already provided a body of data and will, we anticipate, guide the efforts of mutagenesis studies in the future when suitable expression systems become available.