Hot isostatic pressure treatment of 3D printed Ti6Al4V alters surface modifications and cellular response.
Hot isostatic pressure treatment of 3D printed Ti6Al4V alters surface modifications and cellular response.
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DOI：
10.1002/jbm.b.34474
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发表时间：
2020-05
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影响因子：
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通讯作者：
Michael B Berger;Thomas W. Jacobs;B. Boyan;Z. Schwartz
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文献类型：
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作者：
Michael B Berger;Thomas W. Jacobs;B. Boyan;Z. Schwartz
增材制造可用于创建具有不同几何形状和孔隙率的个性化骨科和牙科植入物，旨在模拟骨的形态学特性。这些特性可以减轻应力遮挡，并通过骨长入增加骨整合，但与机加工植入物相比，其代价是疲劳性能降低，以及松散构建颗粒侵蚀的可能性。热等静压（HIP）处理用于提高抗疲劳性;喷砂和酸蚀等植入物表面处理可产生微粗糙度并减少松散颗粒的存在。然而，尚不清楚HIP处理如何影响表面处理和种植体与骨的骨整合。我们制造了两种钛-铝-钒结构，一种具有简单的穿透孔隙率，另一种具有复杂的小梁骨样孔隙率。我们观察到HIP治疗的效果各不相同，并依赖于架构。通过喷砂和酸蚀刻产生的微/介/纳米表面性质在仿生HIP处理的结构上发生改变。将人间充质干细胞（MSC）在+/- HIP制造的构建体上培养，随后进行表面处理。间充质干细胞是敏感的三维结构，表现出更大的成骨分化与复杂的骨小梁样孔隙结构。HIP处理没有改变这些结构的MSC的成骨反应。因此，HIP在种植体骨整合和功能期间可能提供机械和生物学优势。
Additive manufacturing can be used to create personalized orthopedic and dental implants with varying geometries and porosities meant to mimic morphological properties of bone. These qualities can alleviate stress shielding and increase osseointegration through bone ingrowth, but at the expense of reduced fatigue properties compared to machined implants, and potential for loose build particle erosion. Hot isostatic pressure (HIP) treatment is used to increase fatigue resistance; implant surface treatments like grit-blasting and acid-etching create microroughness and reduce the presence of loose particles. However, it is not known how HIP treatment affects surface treatments and osseointegration of the implant to bone. We manufactured two titanium-aluminum-vanadium constructs, one with simple through-and-through porosity and one possessing complex trabecular bone-like porosity. We observed HIP treatment varied in effect and was dependent on architecture. Micro/meso/nano surface properties generated by grit-blasting and acid-etching were altered on biomimetic HIP-treated constructs. Human mesenchymal stem cells (MSCs) were cultured on constructs fabricated +/- HIP and subsequently surface-treated. MSCs were sensitive to 3D-architecture, exhibiting greater osteogenic differentiation on constructs with complex trabecular bone-like porosity. HIP-treatment did not alter the osteogenic response of MSCs to these constructs. Thus, HIP may provide mechanical and biological advantages during implant osseointegration and function.