Transcription Factors Downstream of IL-4 and TGF-β Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry
Transcription Factors Downstream of IL-4 and TGF-β Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry
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DOI：
10.1007/978-1-4939-6877-0_11
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Mikami, Norihisa
中科院分区：
文献类型：
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作者：
Sugimoto, Atsushi;Kawakami, Ryoji;Mikami, Norihisa
产生il -9的Th9细胞是一种新的Th9细胞亚群，参与II型过敏性炎症，如哮喘。在IL-4和tgf - β存在的情况下，Th9细胞可以被幼稚的Th细胞诱导。IL-4和tgf - β的下游信号，包括STAT6、IRF4、Smad和PU.1，也被证实直接介导Th9细胞中IL-9的产生。在本章中，我们描述了流式细胞术、qPCR和western blot分析的方法，以确定IL-4和tgf - β下游这些转录因子的表达或激活。
IL-9-producing Th9 cell is a novel Th cell subset involved in type II allergic inflammations such as asthma. Th9 cells can be induced from naive Th cells in the presence of IL-4 and TGF-beta. It is also well established that downstream signals of IL-4 and TGF-beta, including STAT6, IRF4, Smad, and PU.1, directly mediate IL-9 production in Th9 cells. In this chapter we describe the methods of flow cytometry, qPCR and western blot analysis to determine the expression or activation of these transcription factors downstream of IL-4 and TGF-beta.