BETAview:: a digital β-imaging system for dynamic studies of biological phenomena
BETAview:: a digital β-imaging system for dynamic studies of biological phenomena
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DOI：
10.1016/s0168-9002(01)01724-7
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发表时间：
2002-02-01
影响因子：
1.4
通讯作者：
Russo, P
中科院分区：
文献类型：
--
作者：
Bertolucci, E;Conti, M;Russo, P
我们提出了一种基于半导体像素探测器和单粒子计数芯片的数字放射自显影(DAR)系统，称为BETAview，用于定量分析生物样品中的β辐射示踪剂。该系统能够对随时间变化的生物现象进行实时监测。BETAview既可以配备砷化镓，也可以配备硅半导体像素化探测器。在本文中，我们描述了一个基于硅探测器的组件所获得的结果，该组件厚300针，被分割成×170微米大小的正方形像素。该探测器与欧洲核子研究中心的一个欧洲合作开发的名为MediPix1的低阈值单粒子计数芯片结合在一起。敏感区约为1厘米(2)。对背景噪声和探测效率进行了研究。此外，对放射性标记分子的时间分辨细胞摄取研究也进行了监测。具体地说，我们在体内和实时跟踪了章鱼卵对[C-14]L亮氨酸氨基酸的摄取，证实了先前论文的初步结果。这开启了这种新型半导体DAR系统的生物分子磁学研究领域，其发展(使用MediPix2芯片，256 x 256像素，55微米像素大小)即将到来。(C)2002 Elsevier Science B.V.保留所有权利。
We present a digital autoradiography (DAR) system, named BETAview, based on semiconductor pixel detectors and a single particle counting chip, for quantitative analysis of beta-emitting radioactive tracers in biological samples. The system is able to perform a real time monitoring of time-dependent biological phenomena. BETAview could be equipped either with GaAs or with Si semiconductor pixellated detectors. In this paper, we describe the results obtained with an assembly based on a Si detector, 300 pin thick, segmented into 64 x 64 170 mum size square pixels. The detector is bump-bonded to the low threshold, single particle counting chip named Medipix1, developed by a CERN-based European collaboration. The sensitive area is about 1 cm(2). Studies of background noise and detection efficiency have been performed. Moreover, time-resolved cellular uptake studies with radiolabelled molecules have been monitored. Specifically, we have followed in vivo and in real time, the [C-14]L-leucine amino acid uptake by eggs of Octopus vulgaris confirming the preliminary results of a previous paper. This opens the field of biomolecular kynetic studies with this new class of semiconductor DAR systems, whose evolution (using the Medipix2 chip, 256 x 256 pixels, 55 mum pixel size) is soon to come. (C) 2002 Elsevier Science B.V. All rights reserved.