Rapid nanopore discrimination between single polynucleotide molecules
Rapid nanopore discrimination between single polynucleotide molecules
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DOI：
10.1073/pnas.97.3.1079
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发表时间：
2000-02-01
影响因子：
11.1
通讯作者：
Branton, D
中科院分区：
文献类型：
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作者：
Meller, A;Nivon, L;Branton, D
多种不同的DNA聚合物被电泳驱动通过脂质双层中的α-溶血素通道的纳米孔。在单个多核苷酸的遍历期间，易位持续时间和电流的单通道记录产生了所测试的几种聚合物中的每一种的独特事件模式。来自这种事件模式的统计数据表明，在几种情况下，纳米孔可以区分仅在序列上不同的相似长度和组成的多核苷酸。温度对易位过程影响的研究表明，易位持续时间尺度与T-2相似。事件特征的温度依赖性与某些聚合物形成二级结构的倾向之间存在很强的相关性。由于纳米孔可以快速区分和表征低拷贝数的未标记DNA分子，因此本文所展示的实验方法的改进最终可以提供一种低成本的高通量DNA多核苷酸分析方法。
A variety of different DNA polymers were electrophoretically driven through the nanopore of an alpha-hemolysin channel in a lipid bilayer, Single-channel recording of the translocation duration and current flow during traversal of individual polynucleotides yielded a unique pattern of events for each of the several polymers tested. Statistical data derived from this pattern of events demonstrate that in several cases a nanopore can distinguish between polynucleotides of similar length and composition that differ only in sequence. Studies of temperature effects on the translocation process show that translocation duration scales as similar to T-2. A strong correlation exists between the temperature dependence of the event characteristics and the tendency of some polymers to form secondary structure. Because nanopores can rapidly discriminate and characterize unlabeled DNA molecules at low copy number, refinements of the experimental approach demonstrated here could eventually provide a low-cost high-throughput method of analyzing DNA polynucleotides.