Epstein-Barr virus in the enlarged salivary tissues of patients with IgG4-related disease.
Epstein-Barr virus in the enlarged salivary tissues of patients with IgG4-related disease.
复制标题
IgG4 相关疾病患者增大的唾液组织中的 Epstein-Barr 病毒。
DOI：
10.1002/lary.25153
复制
发表时间：
2015
期刊：
影响因子：
2.6
通讯作者：
Kakehata S
中科院分区：
文献类型：
--
作者：
Furukawa T;Shimotai Y;Ohta N;Ishida A;Kurakami K;Suzuki H;Yamakawa M;Hongo S;Kakehata S
免疫球蛋白G4相关疾病（IgG 4-RD）是最近发现的一种疾病，其特征是高血清IgG 4浓度和产生IgG 4的浆细胞产生，并伴有受累器官的纤维化或硬化变化。我们的目的是澄清的作用，爱泼斯坦巴尔病毒（EBV）在患者与IgG 4-RDs.Study设计和SettingA回顾性临床研究，在山形大学医学院，Yamagata，Japanes.MethodsThe患者组包括四名男性和四名女性，平均年龄为62岁（范围：48-73）。采用免疫组织化学和原位杂交检测IgG 4-RD患者受累唾液腺中IgG 4、潜伏成员蛋白1、EBV核抗原-2和EBV编码RNA的表达。在唾液腺中的EBV DNA的拷贝数也进行了调查，通过真实的时间polymerase chain reaction.ResultsAll患者有硬肿块的唾液腺或泪腺，或两者，双边。所有病例的血清IgG 4浓度均升高（平均值589.1，范围129-1750），在受累唾液腺中观察到IgG 4阳性浆细胞。4例患者在初始唾液腺肿胀后发生了可能危及生命的全身受累。EBV相关分子（EBNA和EBER）在受影响的唾液腺中过表达。EBV DNA的拷贝数在有潜在危及生命的全身受累的患者中显著高于无全身受累的患者（P<0. 05）。结论这些结果表明，EBV DNA的拷贝数可以作为IgG 4-RD的诊断结果，预测潜在危及生命的全身受累。
ObjectiveImmunoglobulin G4‐related disease (IgG4‐RD) is a recently recognized disease entity characterized by high‐serum IgG4 concentration and IgG4‐producing plasma cell production with fibrotic or sclerotic changes in affected organs. We aimed to clarify the roles of Epstein‐Barr virus (EBV) in patients with IgG4‐RDs.Study Design and SettingA retrospective clinical study at the Yamagata University School of Medicine, Yamagata, Japan.MethodsThe patient group consisted of four males and four females with an average age of 62 years (range: 48–73). Expression of IgG4, latent member protein 1, EBV nuclear antigens‐2, and EBV‐encoded RNA in affected salivary glands from patients with IgG4‐RD was examined by using immunohistochemistry and in situ hybridization. The copy number of EBV DNA in the salivary glands was also investigated by real‐time polymerase chain reaction.ResultsAll patients had hard masses in the salivary or lacrimal glands, or both, bilaterally. Serum concentrations of IgG4 were elevated in all cases (mean 589.1, range 129–1750), and IgG4‐positive plasmacytes were observed in the involved salivary glands. Four patients developed potentially life‐threatening systemic involvement after initial salivary gland swelling. EBV‐associated molecules (EBNA and EBER) were overexpressed in the affected salivary glands. The copy number of EBV DNA was significantly higher in patients with potentially life‐threatening systemic involvement than in patients without systemic involvement (P< 0.05).ConclusionThese results suggest that the copy number of EBV DNA could be useful as diagnostic findings in IgG4‐RD to predict potentially life‐threatening systemic involvement.Level of Evidence4.Laryngoscope, 125:2079–2084, 2015