SELECTIVE PHOTOTHERMOLYSIS OF CUTANEOUS PIGMENTATION BY Q-SWITCHED ND-YAG LASER-PULSES AT 1064, 532, AND 355 NM
SELECTIVE PHOTOTHERMOLYSIS OF CUTANEOUS PIGMENTATION BY Q-SWITCHED ND-YAG LASER-PULSES AT 1064, 532, AND 355 NM
复制标题
DOI：
10.1111/1523-1747.ep12277339
复制
发表时间：
1989-07-01
影响因子：
6.5
通讯作者：
DOVER, JS
中科院分区：
文献类型：
--
作者：
ANDERSON, RR;MARGOLIS, RJ;DOVER, JS
皮肤暴露在纳秒激光脉冲下，通过一种被称为选择性光热分解的过程影响色素系统，在这个过程中，黑素体和色素细胞被优先改变。由于黑色素的吸收光谱很宽，这种效应可能发生在穿透组织内部不同深度的波长上，从而可能产生不同的生物反应。通过肉眼、组织学和电镜观察，确定了持续时间为10-12 nsec的单次近紫外（355nm）、可见光（532nm）和近红外（1064nm）脉冲对豚鼠皮肤的影响。色素皮肤的阈值反应是瞬时的灰白色变色，在355,532和1064 nm分别需要011,0.20和1.0 J/cm2。在每个波长下，角化细胞和黑素细胞内的黑素体破裂，细胞质和核发生改变。发生迟发性表皮色素沉着，随后逐渐重新色素沉着。深滤泡细胞仅在532和1064 nm处发生改变，产生永久性白斑。阈值反应的作用谱与选择性光热分解的机理一致。这些数据可能有助于考虑治疗皮肤色素沉着异常或不必要的滤泡色素沉着，或两者兼而有之。
Exposure of skin to nanosecond-domain laser pulses affects the pigmentary system by a process called selective photothermolysis, in which melanosomes and pigmented cells are preferentially altered. Due to the broad absorption spectrum of melanin, this effect may occur with wavelengths that penetrate to vastly different depths within tissue, potentially producing different biologic responses. The effects of single near-ultraviolet (355 nm), visible (532 nm), and near infrared (1064 nm) pulses of 10-12 nsec duration were determined in guinea pig skin using gross, histologic, and electron microscopic observations. Threshold response in pigmented skin was a transient immediate ash-white discoloration, requiring 011, 0.20, and 1.0 J/cm2, at 355, 532, and 1064 nm, respectively. At each wavelength, melanosomes were ruptured within keratinocytes and melanocytes, with cytoplasmic and nuclear alterations. Delayed epidermal depigmentation occurred, followed by gradual repigmentation. Deep follicular cells were altered only at 532 and 1064 nm, which produced permanent leukotrichia. The action spectrum for threshold response was consistent with mechanisms implied by selective photothermolysis. These data may be useful for consideration of treatment for cutaneous pigmentation abnormalities or unwanted follicular pigmentation, or both.