OCCURRENCE OF MULTIPLE ANTIBIOTIC-RESISTANCE AND R-PLASMIDS IN GRAM-NEGATIVE BACTERIA ISOLATED FROM FECALLY CONTAMINATED FRESH-WATER STREAMS IN HONG-KONG
OCCURRENCE OF MULTIPLE ANTIBIOTIC-RESISTANCE AND R-PLASMIDS IN GRAM-NEGATIVE BACTERIA ISOLATED FROM FECALLY CONTAMINATED FRESH-WATER STREAMS IN HONG-KONG
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DOI：
10.1017/s095026880006204x
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发表时间：
1987-06-01
影响因子：
4.2
通讯作者：
MARK, KK
中科院分区：
文献类型：
--
作者：
FRENCH, GL;LING, J;MARK, KK
研究人员对香港新界人口稠密地区的六条淡水溪流的细菌种群进行了研究。这些河流存在相当大的粪便污染，大肠菌群计数高达 105 c.f.u./ml，污染微生物表现出高度普遍的抗生素耐药性和多重耐药性。将水样直接铺板到含抗生素的培养基上，平均 49% 的革兰氏阴性菌对氨苄青霉素耐药，3% 对氯霉素耐药，1% 对庆大霉素耐药。个别地点对这三种药物的耐药率分别高达98%、8%和3%。超过 70% 的菌株对两种或多种抗生素具有耐药性，29% 对五种或以上抗生素耐药，2% 对八种或以上抗生素耐药。总共检测到 98 种抗生素耐药性模式，没有一种模式占主导地位。二十八种革兰氏阴性细菌被鉴定为河流污染物。大肠杆菌是最常见的分离细菌种类，其他常见分离菌包括肠杆菌属、克雷伯氏菌属。和柠檬酸杆菌属，但未检测到肠道病原体。耐药性和多重耐药性的最大流行与大肠杆菌的最严重污染有关。对所选流分离株的分析揭示了以多种不同模式排列的多个质粒带，但多种抗生素耐药性通常是由单个可转移质粒介导的。香港被粪便污染的淡水溪流可能是临床重要细菌的抗生素抗性质粒的储存库。
The bacterial populations of six freshwater streams in populated areas of the Hong Kong New Territories were studied. There is considerable faecal contamination of these streams, with coliform counts as high as 105 c.f.u./ml and the contaminating organisms show a high prevalence of antibiotic resistance and multiple resistance. With direct plating of water samples onto antibiotic-containing media, an average of 49% of the gram-negative bacteria were ampicillin-resistant, 3% chloramphenicol-resistant and 1% gentamicin-resistant. At individual sites resistance to these three drugs was as high as 98%, 8% and 3% respectively. More than 70% of strains were resistant to two or more antibiotics, 29% to five or more and 2% to eight or more. A total of 98 patterns of antibiotic resistance was detected with no one pattern predominating. Twenty-eight gram-negative bacterial species were identified as stream contaminants. Escherichia coli was the commonest bacterial species isolated and other frequent isolates were Enterobacter sp., Klebsiella sp. and Citrobacter sp., but no enteric pathogens were detected. The greatest prevalence of resistance and multiple resistance was associated with the heaviest contamination by E. coli. Analysis of selected stream isolates revealed multiple plasmid bands arranged in many different patterns, but multiple antibiotic resistances were shown to be commonly mediated by single transferable plasmids. Faecally-contaminated freshwater streams in Hong Kong may be reservoirs of antibiotic resistance plasmids for clinically-important bacteria.