Redistribution of Golgi stacks and other organelles during mitosis and cytokinesis in plant cells
Redistribution of Golgi stacks and other organelles during mitosis and cytokinesis in plant cells
复制标题
DOI：
10.1104/pp.124.1.135
复制
发表时间：
2000-09-01
期刊：
影响因子：
7.4
通讯作者：
Staehelin, LA
中科院分区：
文献类型：
--
作者：
Nebenführ, A;Frohlick, JA;Staehelin, LA
我们已经遵循了高尔基体堆栈在有丝分裂和胞质分裂在活烟草BY-2悬浮培养细胞的绿色荧光蛋白标记的大豆α-1，2甘露糖苷酶的装置的重新分配，并与细胞骨架重排和内质网，线粒体和质体的重新分配的结果。在细胞分裂的准备过程中，当高尔基体的一般流动停止时，大约三分之一的外周高尔基体重新分配到核周细胞质，即膜体，从而将内部高尔基体与皮质高尔基体的比例从2：3逆转为3：2。在中期，大约20%的高尔基体聚集在有丝分裂纺锤体附近，类似数量的聚集在质膜下的赤道区域。后一个定位，“高尔基带”，准确地预测了未来的网站细胞分裂，从而形成了一个新的标记后，该地区的preprophase手拆卸。在细胞分裂末期和胞质分裂期间，许多高尔基体堆重新分布在形成细胞板的成膜体周围。在胞质分裂末期，子细胞具有非常相似的高尔基体堆积密度。优先高尔基堆本地化的网站是特定的这个细胞器，并在很大程度上排除线粒体和质体，虽然一些线粒体可以接近成膜体。细胞器的这种分离首先在中期观察到，并持续到胞质分裂完成。不同的本地化的维护不依赖于完整的肌动蛋白丝或微管，虽然有丝分裂纺锤体似乎在组织细胞器的分布模式中发挥了重要作用。在有丝分裂和胞质分裂过程中高尔基体的重新分布与高尔基体重新定位以确保子细胞之间的平等分配以及快速细胞板组装的假设一致。
We have followed the redistribution of Golgi stacks during mitosis and cytokinesis in living tobacco BY-2 suspension culture cells by means of a green fluorescent protein-tagged soybean alpha-1,2 mannosidase, and correlated the findings to cytoskeletal rearrangements and to the redistribution of endoplasmic reticulum, mitochondria, and plastids. In preparation for cell division, when the general streaming of Golgi stacks stops, about one-third of the peripheral Golgi stacks redistributes to the perinuclear cytoplasm, the phragmosome, thereby reversing the ratio of interior to cortical Golgi from 2:3 to 3:2. During metaphase, approximately 20% of all Golgi stacks aggregate in the immediate vicinity of the mitotic spindle and a similar number becomes concentrated in an equatorial region under the plasma membrane. This latter localization, the "Golgi belt," accurately predicts the future site of cell division, and thus forms a novel marker for this region after the disassembly of the preprophase hand. During telophase and cytokinesis, many Golgi stacks redistribute around the phragmoplast where the cell plate is formed. At the end of cytokinesis, the daughter cells have very similar Golgi stack densities. The sites of preferential Golgi stack localization are specific for this organelle and largely exclude mitochondria and plastids, although some mitochondria can approach the phragmoplast. This segregation of organelles is first observed in metaphase and persists until completion of cytokinesis. Maintenance of the distinct localizations does not depend on intact actin filaments or microtubules, although the mitotic spindle appears to play a major role in organizing the organelle distribution patterns. The redistribution of Golgi stacks during mitosis and cytokinesis is consistent with the hypothesis that Golgi stacks are repositioned to ensure equal partitioning between daughter cells as well as rapid cell plate assembly.