Identification and characterization of multiple Spidroin 1 genes encoding major ampullate silk proteins in Nephila clavipes.
Identification and characterization of multiple Spidroin 1 genes encoding major ampullate silk proteins in Nephila clavipes.
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DOI：
10.1111/j.1365-2583.2008.00828.x
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发表时间：
2008-09
影响因子：
2.6
通讯作者：
Marcotte WR Jr
中科院分区：
文献类型：
--
作者：
Gaines WA 4th;Marcotte WR Jr
蜘蛛拖丝主要由称为大壶状腺蛛丝蛋白（MaSp）的蛋白质组成，其由两侧为非重复N-和C-末端结构域的大重复阵列组成。直到最近，几乎没有证据表明两种主要的蛛丝蛋白MaSp 1和MaSp 2都有一个以上的基因编码。在这里，我们报告了推导的N-末端结构域序列的两个不同的MaSp 1基因从Nephila clavipes（MaSp 1A和MaSp 1B）和MaSp 2。所有三个MaSp基因在大壶腹腺中共表达。检索GenBank数据库还发现了两个不同的MaSp 1 C-末端结构域序列。测序证实，这两个MaSp 1基因都存在于所有七个Nephila clavipes蜘蛛检查。这些基因中核苷酸多态性的存在证实了MaSp 1A和MaSp 1B是不同的遗传位点，而不仅仅是同一基因的等位基因。我们已经通过实验确定了所有三个MaSp基因的转录起始位点，并建立了两个MaSp 1 N-和C-末端结构域之间的初步配对。这些新的序列和其他公布的MaSp N-和C-末端结构域序列的系统发育分析表明，重复的MaSp基因可能是广泛的蜘蛛物种。
Spider dragline silk is primarily composed of proteins called major ampullate spidroins (MaSp) that consist of a large repeat array flanked by non-repetitive N- and C-terminal domains. Until recently, there has been little evidence for more than one gene encoding each of the two major spidroin silk proteins, MaSp1 and MaSp2. Here, we report the deduced N-terminal domain sequences for two distinct MaSp1 genes from Nephila clavipes (MaSp1A and MaSp1B) and for MaSp2. All three MaSp genes are co-expressed in the major ampullate gland. A search of the GenBank database also revealed two distinct MaSp1 C-terminal domain sequences. Sequencing confirmed that both MaSp1 genes are present in all seven Nephila clavipes spiders examined. The presence of nucleotide polymorphisms in these genes confirmed that MaSp1A and MaSp1B are distinct genetic loci and not merely alleles of the same gene. We have experimentally determined the transcription start sites for all three MaSp genes and established preliminary pairing between the two MaSp1 N- and C-terminal domains. Phylogenetic analysis of these new sequences and other published MaSp N- and C-terminal domain sequences illustrated that duplications of MaSp genes may be widespread among spider species.
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影响因子：
6.2
作者：
Ittah, Shmulik;Cohen, Shulamit;Gat, Uri
通讯作者：
Gat, Uri
影响因子：
13.8
作者：
Combet, C;Blanchet, C;Deléage, G
通讯作者：
Deléage, G
DOI：
10.1016/s0141-8130(98)00094-4
发表时间：
1999-03-01
影响因子：
8.2
作者：
Madsen, B;Shao, ZZ;Vollrath, F
通讯作者：
Vollrath, F
影响因子：
1.5
作者：
Rasch, EM;Connelly, BA
通讯作者：
Connelly, BA
影响因子：
14.9
作者：
Nakamura, Y;Gojobori, T;Ikemura, T
通讯作者：
Ikemura, T