Isolation and characterization of full‐length chromosomes from non‐culturable plant‐pathogenic Mycoplasma‐like organisms
Isolation and characterization of full‐length chromosomes from non‐culturable plant‐pathogenic Mycoplasma‐like organisms
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非可培养植物病原性支原体样生物体全长染色体的分离和表征
DOI：
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发表时间：
1993
影响因子：
3.6
通讯作者：
B. Kirkpatrick
中科院分区：
文献类型：
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作者：
H. Neimark;B. Kirkpatrick
我们描述了从不可培养的植物病原性支原体样生物（MLO）中分离和鉴定全长染色体。MLO染色体呈圆形，大小（640 - 1185 kbp）不均一。在两个主要的MLO疾病组中，MLO之间的染色体大小范围明显不同，并且在一些相关因子中发生染色体大小多态性。MLO染色体大小与可培养支原体的染色体大小重叠;因此，单独的小基因组大小不能解释MLO不可培养性。与克隆的MLO特异性染色体和16 S rRNA探针杂交，在一些MLO“型”菌株中检测到两条单独的染色体。还证实了似乎是MLO巨质粒的大DNA分子。从几乎任何不可培养的原核生物中表征全长染色体的能力将极大地促进这些困难细菌的分子和遗传分析。
We describe the isolation and characterization of full‐length chromosomes from non‐culturable plant‐pathogenic, mycoplasma‐like organisms (MLOs). MLO chromosomes are circular and their sizes (640 to 1185kbp) are heterogeneous. Divergence in the range of chromosome sizes is apparent between MLOs in the two major MLO disease groups, and chromosome size polymorphism occurs among some related agents. MLO chromosome sizes overlap those of culturable mycoplasmas; consequently, small genome size alone cannot explain MLO non‐culturability. Hybridization with cloned MLO‐specific chromosomal and 16S rRNA probes detected two separate chromosomes in some MLO ‘type’ strains. Large DNA molecules that appear to be MLO megaplasmids were also demonstrated. The ability to characterize full‐length chromosomes from virtually any non‐culturable prokaryote should greatly facilitate the molecular and genetic analysis of these difficult bacteria.
用于检测和扩增真细菌核酸的广泛 DNA 探针。
影响因子：
2.1
作者：
Chen,K;Neimark,H;Rumore,P;Steinman,CR
通讯作者：
Steinman,CR