DNA-mediated engineering of multicomponent enzyme crystals
DNA-mediated engineering of multicomponent enzyme crystals
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DOI：
10.1073/pnas.1503533112
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发表时间：
2015-04-14
影响因子：
11.1
通讯作者：
Mirkin, Chad A.
中科院分区：
文献类型：
--
作者：
Brodin, Jeffrey D.;Auyeung, Evelyn;Mirkin, Chad A.
可预测地控制多个纳米级构建块（特别是那些具有不同化学和物理性质的构建块，例如生物分子和无机纳米颗粒）的共组装的能力，在催化、传感和光子学方面具有深远的影响，但工程化的可推广策略这些颗粒之间的特定接触是一个突出的挑战。在蛋白质的情况下尤其如此，其中可能的粒子间相互作用的类型是众多的，多样的，复杂的。在这里，我们探索的概念交易蛋白质-蛋白质相互作用的DNA-DNA相互作用，以指导组装的两个核酸功能化的蛋白质与不同的表面化学组成的催化活性蛋白质，或蛋白质和DNA修饰的金纳米粒子的组合的六个独特的晶格。在这种策略中使用的DNA-DNA相互作用的可编程性质使我们能够控制晶格对称性和晶胞常数，以及所得晶体的组成和习性。这项研究提供了一个潜在的可推广的策略，用于构建一类独特的材料，利用不同的形态，表面化学和功能的蛋白质组装功能性晶体材料。
The ability to predictably control the coassembly of multiple nanoscale building blocks, especially those with disparate chemical and physical properties such as biomolecules and inorganic nanoparticles, has far-reaching implications in catalysis, sensing, and photonics, but a generalizable strategy for engineering specific contacts between these particles is an outstanding challenge. This is especially true in the case of proteins, where the types of possible interparticle interactions are numerous, diverse, and complex. Herein, we explore the concept of trading protein-protein interactions for DNA-DNA interactions to direct the assembly of two nucleic-acid-functionalized proteins with distinct surface chemistries into six unique lattices composed of catalytically active proteins, or of a combination of proteins and DNA-modified gold nanoparticles. The programmable nature of DNA-DNA interactions used in this strategy allows us to control the lattice symmetries and unit cell constants, as well as the compositions and habit, of the resulting crystals. This study provides a potentially generalizable strategy for constructing a unique class of materials that take advantage of the diverse morphologies, surface chemistries, and functionalities of proteins for assembling functional crystalline materials.