Conjunctival M cells selectively bind and translocate Maackia amurensis leukoagglutinin
Conjunctival M cells selectively bind and translocate Maackia amurensis leukoagglutinin
复制标题
DOI：
10.1016/j.exer.2004.11.005
复制
发表时间：
2005-04-01
影响因子：
3.4
通讯作者：
Phillips, TE
中科院分区：
文献类型：
--
作者：
Meagher, CK;Liu, HS;Phillips, TE
抗原采样M细胞存在于许多粘膜中有组织淋巴组织上方的滤泡相关上皮中。它们在启动粘膜免疫应答中起关键作用，并作为机会性病原体的进入位点。本研究探讨了豚鼠结膜中M细胞的存在。通过筛选12种凝集素和5种糖抗原表位抗体，确定怀槐白细胞凝集素I和II（MAL-I和MAL-II）为潜在的结膜M细胞标记物。然后将生物素化或荧光素缀合的MAL-I滴入体内结膜囊中15-60 min。通过落射荧光立体显微镜、共聚焦扫描激光显微镜以及透射和扫描电子显微镜（TEM和SEM）对样本进行评估。在体内暴露于MAL-I后，观察到覆盖淋巴滤泡的上皮细胞亚群的选择性标记。MAL-I标记仅限于具有稀疏、不规则微绒毛的细胞。发现优先标记MAL-I的细胞在体内暴露60分钟期间内化凝集素。在45分钟的体内孵育过程中，MAL-I被胞吞到充满胞内囊泡的细胞基底外侧膜。本研究表明，豚鼠结膜含有一种具有抗原采样M细胞形态和功能特征的细胞。(c)2004 Elsevier Ltd.保留所有权利。
Antigen-sampling M cells are found in the follicle-associated epithelium above organized lymphoid tissue in many mucosae. They play a key role in initiating the mucosal immune response and act as a site of entry for opportunistic pathogens. This study investigates the presence of M cells in the Guinea pig conjunctiva. Maackia amurensis leukoagglutinin I and II (MAL-I and MAL-II) were identified as potential conjunctival M cell markers based on a screening of 12 lectins and 5 carbohydrate epitope antibodies on aldehyde-fixed follicles. Biotinylated or fluorescein-conjugated MAL-I was then instilled into conjunctival sacs in vivo for 15-60 min. Specimens were assessed by epi-fluorescence stereomicroscopy, confocal scanning laser microscopy and transmission and scanning electron microscopy (TEM and SEM). Selective labelling of a subset of epithelial cells overlying lymphoid follicles was observed following in vivo exposure to MAL-I. MAL-I labelling was restricted to cells with sparse, irregular microvilli. Cells preferentially labelled with MAL-I were found to internalize the lectin during a 60 min in vivo exposure. MAL-I was transcytosed to basolateral membranes of cells filled with intracellular vesicles during a 45 min in vivo incubation. This study demonstrates that the Guinea pig conjunctiva contains a cell with morphological and functional characteristics of antigen-sampling M cells. (c) 2004 Elsevier Ltd. All rights reserved.