Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli
Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli
复制标题
重组大肠杆菌将乙酸盐高效转化为间苯三酚
DOI：
10.1039/c7ra09519h
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发表时间：
2017
期刊：
影响因子：
3.9
通讯作者：
Liu Huizhou
中科院分区：
文献类型：
--
作者：
Xu Xin;Xian Mo;Liu Huizhou
间苯三酚是一种重要的精细化工产品，利用成本较低的原料醋酸酯作为替代碳源，尝试由重组大肠杆菌生产间苯三酚。间苯三酚是通过在工程菌中组装醋酸盐同化途径和间苯三酚生物合成途径而显著产生的。在此基础上，对发酵条件进行了优化，以提高间苯三酚的产量，最高滴度可达554 mg L−1。最后，利用优化后的培养条件进行了间苯三酚的补料分批发酵试验，最高发酵浓度为1.20g L−1。与以葡萄糖为碳源的发酵相比，发酵产率(0.74g/gDCW)和产量(0.18g/g醋酸酯)分别提高了3.2倍和1.6 4倍。因此，该工程菌可直接用于乙酸酯生物合成间苯三酚，具有较好的原子经济性和较低的成本。
Phloroglucinol, an important fine chemical, was attempted to be produced by a recombinant Escherichia coli, using acetate, a less costly feedstock, as a alternative carbon source. Phloroglucinol was significantly produced by assembling an acetate assimilation pathway and phloroglucinol biosynthetic pathway in an engineered Escherichia coli strain. Subsequently, the culture conditions were optimized to enhance phloroglucinol production with a maximum titer of 554 mg L−1. Finally, fed-batch fermentation of phloroglucinol was evaluated using the optimized culture conditions, and reached a maximum concentration of 1.20 g L−1. The productivity (0.74 g per g DCW) and yield (0.18 g per g acetate) increased by 3.20-fold and 1.64-fold, respectively, compared with the data using glucose as the carbon source. Therefore, the engineered E. coli cells can be directly emplored for phloroglucinol biosynthesis from acetate with better atom economy and lower cost.