The Neuropeptides FLP-2 and PDF-1 Act in Concert To Arouse Caenorhabditis elegans Locomotion
The Neuropeptides FLP-2 and PDF-1 Act in Concert To Arouse Caenorhabditis elegans Locomotion
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DOI：
10.1534/genetics.116.192898
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发表时间：
2016-11-01
期刊：
影响因子：
3.3
通讯作者：
Kaplan, Joshua M.
中科院分区：
文献类型：
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作者：
Chen, Didi;Taylor, Kelsey P.;Kaplan, Joshua M.
在幼虫蜕皮期间，秀丽隐杆线虫表现出类似睡眠的状态（称为昏睡），其特征是不进食和深度运动静止。与蜕皮周期相关的运动静止和唤醒的节律模式是由感觉反应性的相互变化介导的，由此唤醒与反应性的增加相关。感觉神经元通过释放神经肽 (PDF-1) 和谷氨酸来激发运动。在这里，我们鉴定了第二种唤醒神经肽（FLP-2）。我们证明 FLP-2 通过食欲素样受体 (FRPR-18) 发挥作用，并且 FLP-2 和 PDF-1 的分泌受到相互正反馈的调节。这些结果表明，两种神经肽之间的正反馈可以稳定所激发的行为状态。
During larval molts, Caenorhabditis elegans exhibits a sleep-like state (termed lethargus) that is characterized by the absence of feeding and profound locomotion quiescence. The rhythmic pattern of locomotion quiescence and arousal linked to the molting cycle is mediated by reciprocal changes in sensory responsiveness, whereby arousal is associated with increased responsiveness. Sensory neurons arouse locomotion via release of a neuropeptide (PDF-1) and glutamate. Here we identify a second arousing neuropeptide (FLP-2). We show that FLP-2 acts via an orexin-like receptor (FRPR-18), and that FLP-2 and PDF-1 secretion are regulated by reciprocal positive feedback. These results suggest that the aroused behavioral state is stabilized by positive feedback between two neuropeptides.