Inhibitory effect of cinnamaldehyde, derived from Cinnamomi cortex, on the growth of influenza A/PR/8 virus in vitro and in vivo
Inhibitory effect of cinnamaldehyde, derived from Cinnamomi cortex, on the growth of influenza A/PR/8 virus in vitro and in vivo
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DOI：
10.1016/j.antiviral.2007.01.003
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发表时间：
2007-04-01
影响因子：
7.6
通讯作者：
Ochiai, H.
中科院分区：
文献类型：
--
作者：
Hayashi, K.;Imanishi, N.;Ochiai, H.
我们研究了反式肉桂醛（CA），一个主要成分的挥发油来自肉桂，对流感病毒A/PR/8在体外和体内的生长的抑制作用。当在感染后（p.i.）在Madin-Darby犬肾细胞中，使用固定剂量的CA（40 μ M），当在感染后3小时开始药物处理时，获得最大抑制作用（对照的29.7%病毒产率）。在相同的处理方案下，CA以剂量依赖性方式（20-200 μ M）抑制病毒生长，并且在200 μ M时，病毒产量降低到检测不到的水平。RT-PCR和SDS-PAGE分析表明，CA在转录后水平抑制病毒蛋白合成。在感染肺适应PR-8病毒的小鼠中，吸入（50 mg/笼/天）和鼻接种（250 μ g/小鼠/天）CA显著增加了8天的存活率，分别为100%和70%，而未处理对照组的存活率为20%。重要的是，吸入CA导致病毒产量减少1个对数的支气管肺泡灌洗液感染后第6天，与未经处理的对照组相比。这些发现可能为含甘草的汉方药物治疗急性呼吸道感染性疾病的经验指征提供进一步的支持。(c)2007 Elsevier B. V.保留所有权利。
We have investigated the inhibitory effect of trans-cinnamaldehyde (CA), one of the principal constituents of essential oil derived from Cinnamomi cortex, on the growth of influenza A/PR/8 virus in vitro and in vivo. When 1-h drug treatment was initiated at various times post-infection (p.i.) in Madin-Darby canine kidney cells using a fixed dose of CA (40 mu M), the maximum inhibitory effect (29.7% virus yield of control) was obtained when drug treatment was started at 3 h p.i. Under the same treatment schedule, CA inhibited the virus growth in a dose-dependent manner (20-200 mu M), and, at 200 mu M, the virus yield was reduced to an undetectable level. RT-PCR and SDS-PAGE analyses showed that CA inhibited viral protein synthesis at the post-transcriptional level. In mice infected with the lung-adapted PR-8 virus, inhalation (50 mg/cage/day) and nasal inoculation (250 mu g/mouse/day) of CA significantly increased survival rates on the 8 days to 100% and 70%, respectively, in contrast to a survival rate of 20% in the untreated control group. Importantly, inhalation of CA caused virus yield reduction by 1 log in bronchoalveolar lavage fluid on day 6 after infection, compared with that of the untreated control group. These findings might provide further support to the empirical indication of Cinnamomi cortex-containing Kampo medicines for acute respiratory infectious diseases. (c) 2007 Elsevier B.V. All rights reserved.