Expression and Production of the CXC Chemokine Growth-Related Oncogene-α by Human Eosinophils1
Expression and Production of the CXC Chemokine Growth-Related Oncogene-α by Human Eosinophils1
复制标题
人嗜酸性粒细胞表达和产生 CXC 趋化因子生长相关癌基因-α1
作者：
Terese Persson;Pia Andersson;A. Bjartell;J. Calafat;A. Egesten
炎症部位可见嗜酸性粒细胞和中性粒细胞。然而，他们的角色并不明确。此外，在一些肿瘤性疾病中，嗜酸性粒细胞渗入肿瘤组织。在本研究中，我们发现人嗜酸性粒细胞可以产生大量的中性粒细胞激活的趋化因子生长相关癌基因(GRO-α)。嗜酸性粒细胞在含有晶体的特殊颗粒(190pg/2×10~6细胞)中显示有预先形成的Gro-α。在孵育过程中，Gro-α基因的表达显著增加。在细胞密度较低时，加入肿瘤坏死因子-α或IL-1β可增加嗜酸性粒细胞产生Gro-α，而在较高细胞密度时则不是这样。嗜酸性粒细胞自身可产生肿瘤坏死因子-α，中和抗肿瘤坏死因子-α的抗体可显著抑制Gro-α的产生。这表明，当上调Gro-α基因表达时，肿瘤坏死因子-α的自分泌和旁分泌效应可能是重要的。相反，干扰素-γ，一种典型的Th1型细胞因子，下调了Gro-α的表达。这在炎症消退过程中可能很重要，但也提示嗜酸性粒细胞在不同的炎症环境中扮演不同的角色。在结节硬化型霍奇金病中，肿瘤浸润性嗜酸性粒细胞与预后不良有关。肿瘤组织中的嗜酸性粒细胞大量表达Gro-α。在肿瘤组织中也检测到Gro-α受体CXCR2，这表明嗜酸性粒细胞与肿瘤之间存在相互作用。我们的发现表明，嗜酸性粒细胞可以通过招募携带CXCR2的细胞来促进炎症。此外，嗜酸性粒细胞的这一特征表明这些细胞在某些肿瘤的生物学中发挥了作用。
Eosinophils are seen together with neutrophils at sites of inflammation. However, their roles are not clear. In addition, eosinophils infiltrate tumor tissue in some neoplastic diseases. In this study, we show that large amounts of the neutrophil-activating CXC chemokine growth-related oncogene (GRO)-α can be produced by human eosinophils. Eosinophils showed presence of preformed GRO-α in the crystalloid-containing specific granules (190 pg/2 × 106 cells). During incubation, a strong increase in GRO-α gene expression was seen. At a low cell density, addition of TNF-α or IL-1β increased the production of GRO-α in eosinophils, which was not the case at a higher cell density. Eosinophils can produce TNF-α themselves, and neutralizing Abs against TNF-α significantly inhibited GRO-α production. This suggests that autocrine and paracrine effects from TNF-α can be important when up-regulating GRO-α gene expression. In contrast, IFN-γ, a prototypic Th1-cytokine, down-regulated expression of GRO-α. This may be important during resolution of inflammation but also suggests different roles for eosinophils depending on the inflammatory context. Tumor-infiltrating eosinophils in Hodgkin’s disease of the nodular sclerosing type are associated with a poor prognosis. Eosinophils from such tumor tissue showed an abundant expression of GRO-α. The GRO-α receptor CXCR2 was also detected in tumor tissue, proposing interactions between eosinophils and the tumor. Our findings suggest that eosinophils can promote inflammation through recruitment of CXCR2-bearing cells. In addition, this feature of the eosinophils indicates a role for these cells in the biology of certain tumors.
霍奇金病伴嗜酸性粒细胞增多的 Reed-Sternberg 细胞中白细胞介素 5 信使 RNA 的检测。
影响因子：
20.3
作者：
Samoszuk,M;Nansen,L
通讯作者：
Nansen,L
正常外周血嗜酸性粒细胞持续产生 IL-4 和 IL-10，并刺激产生 IL-8。
DOI：
--
发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Nakajima,H;Gleich,GJ;Kita,H
通讯作者：
Kita,H
影响因子：
4.8
作者：
Wong, DTW;Bowen, SM;Weller, PF
通讯作者：
Weller, PF