Spindle and kinetochore morphology of Dictyostelium discoideum.
Spindle and kinetochore morphology of Dictyostelium discoideum.
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DOI：
10.1083/jcb.68.1.113
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发表时间：
1976-01
期刊：
影响因子：
--
通讯作者：
Moens PB
中科院分区：
文献类型：
--
作者：
Moens PB
盘基网柄藻（Dictyosteelanddiscoideum，n = 7）的染色体分裂中期纺锤体长2 μ m。它由大约20个微管组成，这些微管在纺锤体极体之间似乎是连续的，并且在纺锤体的每一端都有大约20个染色体微管。在分裂后期，纺锤体伸长，染色体微管缩短。纺锤体的长度和结构在这个阶段表明，延长是由伸长以及平行滑动的非染色体微管。在有丝分裂过程中，核被膜大部分保持完整，当纺锤体长6妈妈时，通过内侧收缩发生核分离。当纺锤体为10 μ m长时，发生胞质分裂。在那个时候，动粒的大小增加了一倍。在间期，纺锤体磁极体从细胞核分离到0.7 μ m的距离，它返回在下一个前期开始时，它成为功能上的双重，从而开始形成一个中心纺锤体。在比较细胞型黏菌PolySphondyelium violaceum和D. discoideum，几个相似之处和一些差异是显而易见的。
The metaphase spindle of haploid Dictyostelium discoideum (n = 7) is 2 mum long. It consists of some 20 microtubules which seem continuous between the spindle pole bodies and there are about 20 chromosomal microtubules at each end of the spindle. During anaphase the central spindle elongates and the chromosomal microtubules shorten. The spindle length and structure at this stage suggests that lengthening is caused by elongation as well as parallel sliding of the nonchromosomal microtubules. The nuclear envelope remains mostly intact during mitosis, and nuclear separation through medial constriction takes place when the spindle is 6 mum long. Cytokinesis occurs when the spindle is 10 mum long. At that time the kinetochores double in size. During interphase, the spindle pole body separates from the nucleus to a distance of 0.7 mum, and it returns at the onset of the next prophase when it becomes functionally double, thereby starting the formation of a central spindle. When comparing mitosis in the cellular slime molds Polysphondylium violaceum and D. discoideum, several similarities and some differences are apparent.