ULTRASOUND CONTRAST AGENTS NUCLEATE INERTIAL CAVITATION IN-VITRO
ULTRASOUND CONTRAST AGENTS NUCLEATE INERTIAL CAVITATION IN-VITRO
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DOI：
10.1016/0301-5629(95)93252-u
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发表时间：
1995-01-01
影响因子：
2.9
通讯作者：
THOMAS, RM
中科院分区：
文献类型：
--
作者：
MILLER, DL;THOMAS, RM
一些超声造影剂含有稳定的气体体，本研究旨在确定这些试剂是否可以为惯性空化提供核，通过暴露于 2.17-、2.95-或 3.8-MHz 超声后测量声化学过氧化氢来检测和评估惯性空化，通过真空脱气从旋转管暴露室和磷酸盐缓冲盐水中去除空化核，获得非空化系统通过过滤，以 10(-2)、10(-3) 或 10(-4) 稀释液添加 Albunex(R)，或以 2 mg mL(-1)、0.2 mg mL(-1) 或 0.02 mg mL(-1) 添加 Levovist(R)，所有这些都在 0.41 MPa 或更高空间峰值压力振幅下暴露 5 分钟，在 2.17 MHz 超声下产生显着的 H2O2 。 0.25 秒开启和关闭，转速为 60 rpm，不旋转管子实际上消除了 H2O2 的产生。对于 2.5 分钟的连续暴露，两种试剂在 2.95 MHz 暴露、0.58 MPa 或更高压力下都会引发大量 H2O2 产生，但在 3.8 MHz 暴露、高达 1.16 MPa 时则不会产生大量 H2O2。因此，基于气泡的超声造影剂似乎能够为旋转管曝光系统中的惯性空化提供核。
Some ultrasound contrast agents contain stable bodies of gas, and this study was undertaken to determine if these agents could provide nuclei for inertial cavitation, Inertial cavitation was detected and assessed by the measurement of the sonochemical hydrogen peroxide after exposure to 2.17-, 2.95- or 3.8-MHz ultrasound, A noncavitating system was obtained by removing cavitation nuclei from the rotating tube exposure chambers by vacuum degassing, and from the phosphate-buffered saline medium by filtering, Albunex(R) added at 10(-2), 10(-3) or 10(-4) dilutions, or Levovist(R) added at 2 mg mL(-1), 0.2 mg mL(-1) or 0.02 mg mL(-1) all initiated significant H2O2 production for 2.17-MHz ultrasound at 0.41 MPa or higher spatial peak pressure amplitude for 5 min exposure gated at 0.25 s on and off with 60-rpm rotation, Not rotating the tube virtually eliminated H2O2 production. For 2.5-min continuous exposure, both agents initiated significant H2O2 production for 2.95-MHz exposure at 0.58 MPa or higher, but not for 3.8-MHz exposure up to 1.16 MPa. Bubble-based ultrasound contrast agents therefore appear to be able to provide nuclei for inertial cavitation in the rotating tube exposure system.