Preparation and Biocompatibility Evaluation of PEG-PLL/RGD-PEG-DSPE/Phospholipid/CaP Nanoparticles
Preparation and Biocompatibility Evaluation of PEG-PLL/RGD-PEG-DSPE/Phospholipid/CaP Nanoparticles
复制标题
PEG-PLL/RGD-PEG-DSPE/磷脂/CaP纳米粒子的制备及生物相容性评价
DOI：
10.1166/jbn.2018.2460
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发表时间：
2018-01-01
影响因子：
2.9
通讯作者：
He, Zelai
中科院分区：
文献类型：
--
作者：
Huang, Jingwen;Zhang, Xiangyu;He, Zelai
成功地合成了(精氨酸-甘氨酸-天冬氨酸)-甲氧基聚乙烯glycol-(1，2-distearoyl-sn-glycero-3-phosphoethanolaMine-N)(简称：RGDPEG2000DSPE或RGDPd)，并用氢核磁共振和飞行时间质谱仪对其进行了确证。采用一种新颖、简单的方法在室温下制备了glycol-poly-L-lysine/RGD-PD/phospholipid/calcium聚乙二醇磷酸盐纳米粒(PEGPLL/RGDPd/PL/CAP或MNPs)。透射电子显微镜分析表明，所制得的MNPs为大小均匀的球形，直径约为30 nm，表面光滑。热重分析(TGA)表明，聚乙二醇-PLL/RGD-Pd/PL胶束被包裹在帽壳中。MNPs对溶血、凝血、心脏氧化应激、炎症反应和DNA损伤几乎没有影响，表明在体外和体内的细胞毒性可以忽略不计。在斑马鱼身上的实验表明，在胚胎发育过程中，MNPs不影响成活率和心跳频率，也不会诱导畸形和细胞凋亡。综上所述，这些结果表明新开发的MNPs具有良好的生物相容性和作为药物和基因载体的巨大潜力。
(Arginine-Glycine-Aspartic)-methoxy polyethylene glycol-(1,2-distearoyl-sn-glycero-3-phosphoethanolaMine-N) (abbreviation: RGD-PEG2000-DSPE or RGD-PD) was successfully synthesized and verified by H-1-NMR and MALDI-TOF MS. Polyethylene glycol-poly-L-lysine/RGD-PD/phospholipid/calcium phosphate nanoparticles (PEG-PLL/RGD-PD/PL/CaP NPs or MNPs) were prepared using a novel, simple method conducted at room temperature. Transmission electron microscopy (TEM) analysis showed that the MNPs were spheres of uniform size, with a diameter of similar to 30 nm, and smooth surface. Thermogravimetric analysis (TGA) revealed that the PEG-PLL/RGD-PD/PL micelle was packed in the CaP shell. MNPs had little effect on hemolysis, coagulation, cardiac oxidative stress, inflammatory response and DNA damage, indicating negligible cytotoxicity in vitro and in vivo. Experiments in Zebrafish indicated that the MNPs neither affected the survival rate and heartbeat rate, nor induced malformation and apoptosis during embryogenesis. In conclusion, these results demonstrate that the newly-developed MNPs have good biocompatibility and a great potential as drug and gene carrier.