Multiple genotypes of enterovirus G carrying a papain-like cysteine protease (PL-CP) sequence circulating on two pig farms in Japan: first identification of enterovirus G10 carrying a PL-CP sequence
Multiple genotypes of enterovirus G carrying a papain-like cysteine protease (PL-CP) sequence circulating on two pig farms in Japan: first identification of enterovirus G10 carrying a PL-CP sequence
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DOI：
10.1007/s00705-020-04816-y
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发表时间：
2020-09-19
影响因子：
2.7
通讯作者：
Nagai, Makoto
中科院分区：
文献类型：
--
作者：
Sekiguchi, Yuya;Nagata, Ayaka;Nagai, Makoto
在两个猪场检测到携带木瓜蛋白酶样半胱氨酸蛋白酶（PL-CP）序列的肠道病毒G（EV-G）的两种和三种基因型，并根据VP1序列分别分为基因型G1和G10以及G1、G8和G17。首次检测到带有PL-CP序列的G10 EV-G病毒。 P2 和 P3 区域的系统发育分析按具有高度序列相似性的农场对病毒进行分组。此外，在 2A 区域检测到明显的重组断点，表明含有 PL-CP EV-G 的菌株通过农场中多个循环 EV-G 基因型之间的重组事件获得了序列多样性。
Two and three genotypes of enterovirus G (EV-G) carrying a papain-like cysteine protease (PL-CP) sequence were detected on two pig farms and classified into genotypes G1 and G10, and G1, G8, and G17, respectively, based on VP1 sequences. A G10 EV-G virus bearing a PL-CP sequence was detected for the first time. Phylogenetic analysis of the P2 and P3 regions grouped the viruses by farm with high sequence similarity. Furthermore, clear recombination break points were detected in the 2A region, suggesting that PL-CP EV-G-containing strains gained sequence diversity through recombination events among the multiple circulating EV-G genotypes on the farms.