Gene mutation analysis of sinonasal lymphomas in Indonesia.
Gene mutation analysis of sinonasal lymphomas in Indonesia.
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DOI：
10.3892/or.15.5.1257
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发表时间：
2006-05
期刊：
影响因子：
4.2
通讯作者：
A. Kurniawan;T. Hongyo;E. Hardjolukito;M. Ham;T. Takakuwa;R. Kodariah;Y. Hoshida;T. Nomura;K. Aozasa
中科院分区：
文献类型：
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作者：
A. Kurniawan;T. Hongyo;E. Hardjolukito;M. Ham;T. Takakuwa;R. Kodariah;Y. Hoshida;T. Nomura;K. Aozasa
鼻腔淋巴瘤包括NK/T细胞（NKTCL）型和B细胞型，具有独特的地理发育。在这项研究中，使用聚合酶链式反应 (PCR)-单链构象多态性 (SSCP) 分析了来自印度尼西亚的 41 例鼻窦淋巴瘤（27 例 NKTCL 型和 14 例 B 细胞型）的 p53、K-ras、c-kit、β-catenin 和 bak 基因的突变，然后进行直接测序。 EBER-1探针原位杂交研究显示，85%的NKTCL病例EBV阳性，但没有一种B细胞类型EBV阳性。 NKTCL 中 p53、K-ras、c-kit、β-catenin 和 bak 基因的突变频率分别为 62.9%、0%、11.1%、18.5% 和 25.9%，B 细胞病例中分别为 71.4%、0%、23.1%、21.4% 和 57.1%，表明所有基因的突变频率均为B 细胞中的值高于 NKTCL 病例中的值。这些发现表明，基因突变可能是 B 细胞淋巴瘤的驱动力，而 EBV 感染和基因突变的结合则有助于印度尼西亚 NKTCL 的发展。
Sinonasal lymphomas comprise NK/T-cell (NKTCL) type and B-cell type with unique geographical development. In this study, mutations of p53, K-ras, c-kit, beta-catenin, and bak gene were analyzed using polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP) followed by direct sequencing in 41 sinonasal lymphomas (27 NKTCL and 14 B-cell type) from Indonesia. In situ hybridization study with EBER-1 probe revealed that 85% of NKTCL cases were EBV positive, but none of B-cell type was EBV positive. Frequency of mutations in p53, K-ras, c-kit, beta-catenin, and bak gene was 62.9%, 0%, 11.1%, 18.5%, and 25.9%, respectively, in NKTCL, and 71.4%, 0%, 23.1%, 21.4%, and 57.1%, respectively, in B-cell cases, showing that mutation frequency in all genes was higher in B-cell than in NKTCL cases. These findings suggest that gene mutations might be the driving-force for B-cell lymphoma, whereas combined EBV infection and gene mutations contribute to NKTCL development in Indonesia.