Erythrocyte antioxidant enzymes in multiple sclerosis and the effect of hyperbaric oxygen
Erythrocyte antioxidant enzymes in multiple sclerosis and the effect of hyperbaric oxygen
复制标题
多发性硬化症中的红细胞抗氧化酶和高压氧的作用
DOI：
10.1007/bf00964377
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发表时间：
1984
影响因子：
4.4
通讯作者：
D. Davidson
中科院分区：
文献类型：
--
作者：
M. S. Hunter;M. Lao;S. Burtles;D. Davidson
多发性硬化症患者红细胞过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶活性与正常人无显著差异，而超氧化物歧化酶活性降低(P<0.05)。在严格控制的条件下(过氧化应激试验)，用10 mM的H_2O_2孵育红细胞后，对脂质过氧化产物丙二醛的测定表明，MS红细胞在体外对H_2O_2诱导的脂质过氧化反应的敏感性显著降低(P<0.001)。这一发现表明，MS红细胞中一种内源性抗氧化剂的水平可能会升高，可能是维生素E。经高压氧处理后，MS红细胞过氧化氢酶活性显著(P<；0.01)提高2-6倍。
The activities of catalase, glutathione peroxidase, and glutathione reductase, were not significantly different from normal whereas that of superoxide dismutase was decreased (P<0.05) in erythrocytes from patients with multiple sclerosis. Assay of the lipid peroxidation product, malondialdehyde, after incubation of erythrocytes with 10 mM H2O2under carefully controlled conditions (peroxide stress test) demonstrated that MS erythrocytes are significantly (P<0.001) less susceptible to H2O2-induced lipid peroxidation in vitro. This finding suggests that the level of an endogenous antioxidant, possibly vitamin E, may be elevated in MS red cells. After treatment with hyperbaric O2, the activity of MS erythrocyte catalase is significantly (P<0.01) elevated by 2–6-fold.