Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes.
Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes.
复制标题
复制蛋白 A 与酵母 DNA 修复和 DNA 代谢基因中的调节元件结合。
DOI：
10.1073/pnas.92.11.4907
复制
发表时间：
1995
影响因子：
11.1
通讯作者：
Samson,L
中科院分区：
文献类型：
--
作者：
Singh,KK;Samson,L
酿酒酵母通过阻止细胞周期进程（从而阻止受损染色体的复制和分离）和诱导众多基因的表达来响应 DNA 损伤，其中一些基因参与 DNA 修复、DNA 复制和 DNA 代谢。 DNA 损伤剂诱导酿酒酵母 3-甲基腺嘌呤 DNA 糖基化酶修复基因 (MAG) 需要 MAG 启动子中的一个上游激活序列 (UAS) 和两个上游抑制序列（URS1 和 URS2）。与 MAG URS 元件相似的序列存在于至少 11 个其他酿酒酵母 DNA 修复和代谢基因中。复制蛋白 A (Rpa) 被称为单链 DNA 结合蛋白，参与 DNA 复制的起始和延伸步骤、核苷酸切除修复和同源重组。我们现在证明 MAG URS1 和 URS2 元件形成相似的双链、序列特异性、DNA-蛋白质复合物，并且两种复合物都含有 Rpa。此外，Rpa 似乎可以结合在 11 个其他 DNA 修复和 DNA 代谢基因上游发现的 MAG URS1 样元件。这些结果使我们推测Rpa可能参与许多DNA修复和DNA代谢基因的调节。
Saccharomyces cerevisiae responds to DNA damage by arresting cell cycle progression (thereby preventing the replication and segregation of damaged chromosomes) and by inducing the expression of numerous genes, some of which are involved in DNA repair, DNA replication, and DNA metabolism. Induction of the S. cerevisiae 3-methyladenine DNA glycosylase repair gene (MAG) by DNA-damaging agents requires one upstream activating sequence (UAS) and two upstream repressing sequences (URS1 and URS2) in the MAG promoter. Sequences similar to the MAG URS elements are present in at least 11 other S. cerevisiae DNA repair and metabolism genes. Replication protein A (Rpa) is known as a single-stranded-DNA-binding protein that is involved in the initiation and elongation steps of DNA replication, nucleotide excision repair, and homologous recombination. We now show that the MAG URS1 and URS2 elements form similar double-stranded, sequence-specific, DNA-protein complexes and that both complexes contain Rpa. Moreover, Rpa appears to bind the MAG URS1-like elements found upstream of 11 other DNA repair and DNA metabolism genes. These results lead us to hypothesize that Rpa may be involved in the regulation of a number of DNA repair and DNA metabolism genes.