Investigation of Cyprinid herpesvirus-3 genetic diversity by a multi-locus variable number of tandem repeats analysis
Investigation of Cyprinid herpesvirus-3 genetic diversity by a multi-locus variable number of tandem repeats analysis
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DOI：
10.1016/j.jviromet.2011.03.002
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发表时间：
2011-05-01
影响因子：
3.1
通讯作者：
Maskur, Maskur
中科院分区：
文献类型：
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作者：
Avarre, Jean-Christophe;Madeira, Jean-Paul;Maskur, Maskur
鲤鱼疱疹病毒3型（CyHV-3），又称锦鲤疱疹病毒（KHV），是引起鲤鱼（Cyprinus carpi carpio）和锦鲤（Cyprinus carpio koi）高死亡率的主要病毒。三个CyHV-3分离株的完整基因组显示出超过99%的DNA序列同一性，其中大部分差异位于短串联重复序列，也称为VNTR（可变数目串联重复序列）。通过靶向这些变异，选择8个位点通过多位点VNTR分析（MLVA）对CyHV-3进行基因分型。连续体内感染后获得的CyHV-3菌株表现出相同的MLVA曲线，而来自体外传代6次和82次的单一分离株的样品在8个位点中的2个中表现出突变，表明VNTR的遗传进化速率相对较慢。该方法随后应用于在印度尼西亚、法国和荷兰收集的38个样品。在全球范围内，分离物分为两个主要的遗传簇，每一个分为两个亚组，包括CyHV-3-U/I或CyHV-3-J。有趣的是，印尼株是相当遥远的CyHV-3-J分离。本研究的结果表明，这些VNTR分子标记是有效的，在估计之间的CyHV-3分离株的遗传多样性，因此，适合于进一步的分子流行病学研究。(C)2011 Elsevier B. V.保留所有权利。
Cyprinid herpesvirus-3 (CyHV-3), or koi herpesvirus (KHV), is responsible for high mortalities in aquaculture of both common carp (Cyprinus carpi carpio) and koi carp (Cyprinus carpio koi) worldwide. The complete genomes of three CyHV-3 isolates showed more than 99% of DNA sequence identity, with the majority of differences located in short tandem repeats, also called VNTR (variable number of tandem repeats). By targeting these variations, eight loci were selected for genotyping CyHV-3 by multiple locus VNTR analysis (MLVA). CyHV-3 strains obtained after sequential in vivo infections exhibited identical MLVA profiles, whereas samples originating from a single isolate passaged 6 and 82 times in vitro exhibited mutations in two of the eight loci, suggesting a relatively slow genetic evolution rate of the VNTRs. The method was subsequently applied on 38 samples collected in Indonesia, France and the Netherlands. Globally, the isolates grouped in two main genetic clusters, each one divided in two subgroups including either CyHV-3-U/I or CyHV3-J. Interestingly, Indonesian strains were rather distant from CyHV-3-J isolate. The results of the present study indicate that these VNTR molecular markers are efficient in estimating the genetic diversity among CyHV-3 isolates and are therefore suitable for further molecular epidemiological studies. (C) 2011 Elsevier B.V. All rights reserved.