CDK-dependent nuclear localization of B-cyclin Clb1 promotes FEAR activation during meiosis I in budding yeast.
CDK-dependent nuclear localization of B-cyclin Clb1 promotes FEAR activation during meiosis I in budding yeast.
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DOI：
10.1371/journal.pone.0079001
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Arumugam P
中科院分区：
文献类型：
--
作者：
Tibbles KL;Sarkar S;Novak B;Arumugam P
细胞周期蛋白依赖性蛋白激酶(CDK)是真核生物细胞周期的主要调节因子。CDK的活性受其调节亚单位细胞周期蛋白的存在、翻译后修饰和空间定位的调节。在芽殖酵母中，B-细胞周期蛋白Clb1在减数分裂I过程中被磷酸化并定位于细胞核，但Clb1的S磷酸化和核定位及其相互依赖的功能意义尚不清楚。在这篇文章中，我们证明了减数分裂特异性的Clb1的磷酸化需要它进入细胞核，而不是相反。虽然Clb1的磷酸化依赖于CDK和类似Polo的激酶CDC5的活性，但它的核定位需要CDK而不是CDC5的活性。此外，我们还表明，减数分裂过程中Clb1核定位的增加增强了FREAT(CDC 14早期后期释放)途径的激活。我们讨论了我们的结果对于调节减数分裂I的退出的意义。
Cyclin-dependent kinases (CDK) are master regulators of the cell cycle in eukaryotes. CDK activity is regulated by the presence, post-translational modification and spatial localization of its regulatory subunit cyclin. In budding yeast, the B-cyclin Clb1 is phosphorylated and localizes to the nucleus during meiosis I. However the functional significance of Clb1's phosphorylation and nuclear localization and their mutual dependency is unknown. In this paper, we demonstrate that meiosis-specific phosphorylation of Clb1 requires its import to the nucleus but not vice versa. While Clb1 phosphorylation is dependent on activity of both CDK and polo-like kinase Cdc5, its nuclear localization requires CDK but not Cdc5 activity. Furthermore we show that increased nuclear localization of Clb1 during meiosis enhances activation of FEAR (Cdc Fourteen Early Anaphase Release) pathway. We discuss the significance of our results in relation to regulation of exit from meiosis I.
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DOI：
10.1083/jcb.200812022
发表时间：
2009-02-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mohl DA;Huddleston MJ;Collingwood TS;Annan RS;Deshaies RJ
通讯作者：
Deshaies RJ
影响因子：
64.5
作者：
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通讯作者：
Uhlmann, Frank
影响因子：
10.5
作者：
Benjamin, KR;Zhang, C;Herskowitz, I
通讯作者：
Herskowitz, I
影响因子：
56.9
作者：
Azzam, R;Chen, SL;Deshaies, RJ
通讯作者：
Deshaies, RJ
影响因子：
2.9
作者：
Moore, Michael;Shin, Marcus E.;Winter, Edward
通讯作者：
Winter, Edward