Modulation of trigeminal spinal subnucleus caudalis neuronal activity following regeneration of transected inferior alveolar nerve in rats
Modulation of trigeminal spinal subnucleus caudalis neuronal activity following regeneration of transected inferior alveolar nerve in rats
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DOI：
10.1152/jn.00794.2007
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发表时间：
2008-05-01
影响因子：
2.5
通讯作者：
Iwata, Koichi
中科院分区：
文献类型：
--
作者：
Saito, Kimiko;Hitomi, Suzuro;Iwata, Koichi
大鼠下牙槽神经切断再生后三叉神经脊束核尾侧亚核神经元活动的调节。为了阐明再生的下齿槽神经（IAN）支配的面部异常疼痛的神经元机制，在IAN切断的大鼠中，研究了伤害性行为、将荧光金（FG）注射到颏部皮肤后的三叉神经节神经元标记以及三叉神经脊束尾侧亚核（Vc）神经元特性。机械逃避阈值在IAN横断后3天显著升高，在14天显著降低，而头回缩潜伏期在IAN横断后3、14和60天显著延长。FG标记的神经节神经元的数量显着减少，在3天后IAN横断，但在14和60天增加。与幼稚大鼠相比，IAN横断后14和60天，具有背景（BG）活动的宽动态范围（WDR）神经元的数量显著增加，并且在这两个时间点，具有不规则爆发BG活动的WDR和低阈值机械感受（LTM）神经元的数量增加。机械诱发反应显着更大的WDR和LTM神经元IAN横断后14天相比，幼稚大鼠。与未处理大鼠相比，在横断后14天，WDR神经元的热和冷诱发反应显着降低。机械感受野也显着更大的宽DR和LTM神经元在14和60天后IAN横断。这些结果表明，这些变化可能参与了机械异常性疼痛的发展，在皮肤区域的神经支配的再生IAN。
Modulation of trigeminal spinal subnucleus caudalis neuronal activity following regeneration of transected inferior alveolar nerve in rats. To clarify the neuronal mechanisms of abnormal pain in the face innervated by the regenerated inferior alveolar nerve (IAN), nocifensive behavior, trigeminal ganglion neuronal labeling following Fluorogold (FG) injection into the mental skin, and trigeminal spinal subnucleus caudalis (Vc) neuronal properties were examined in rats with IAN transection. The mechanical escape threshold was significantly higher at 3 days and lower at 14 days after IAN transection, whereas head withdrawal latency to heat was significantly longer at 3, 14, and 60 days after IAN transection. The number of FG-labeled ganglion neurons was significantly reduced at 3 days after IAN transection but increased at 14 and 60 days. The number of wide dynamic range (WDR) neurons with background (BG) activity was significantly higher at 14 and 60 days after IAN transection compared with naive rats, and the number of WDR and low-threshold mechanoreceptive (LTM) neurons with irregularly bursting BG activity was increased at these two time points. Mechanically evoked responses were significantly larger in WDR and LTM neurons 14 days after IAN transection compared with naive rats. Heat- and cold-evoked responses in WDR neurons were significantly lower at 14 days after transection compared with naive rats. Mechanoreceptive fields were also significantly larger in WDR and LTM neurons at 14 and 60 days after IAN transection. These findings suggest that these alterations may be involved in the development of mechanical allodynia in the cutaneous region innervated by the regenerated IAN.