Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers.

Rapid Detection of Rongalite via a Sandwich Lateral Flow Strip Assay Using a Pair of Aptamers.
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使用一对适体通过夹心侧流试纸条检测快速检测雕白粉

DOI:
10.1186/s11671-018-2709-9
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发表时间:
2018-09-24
影响因子:
--
通讯作者:
Han Q
Han Q
中科院分区:
材料科学3区
文献类型:
--
作者:
Li J;Jing L;Song Y;Zhang J;Chen Q;Wang B;Xia X;Han Q

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使用一对金纳米颗粒 (AuNP) 功能化的适体进行夹心侧流试纸条测定 (LFSA),旨在评估农产品中雕白粉的存在情况。更具体地说,将生物素标记的初级 A09 适体固定在链霉亲和素包被的膜上,并开发了与 AuNP 缀合的次级 B09 适体,分别作为捕获探针和信号探针。该系统只需观察 LFSA 对照和测试线的颜色变化,即可成功直接检测食品样品中浓度低至 1 µg/mL 的雕白粉。
A sandwich lateral flow strip assay (LFSA) using a couple of aptamers functionalized with gold nanoparticles (AuNPs) was designed to assess the presence of rongalite in agrifood products. More specifically, a biotin-labeled primary A09 aptamer immobilized on a streptavidin-coated membrane and a secondary B09 aptamer conjugated with AuNPs were developed as capturing and signaling probes, respectively. This system allows the successful and direct detection of rongalite in food samples with concentrations as low as 1 μg/mL, simply by observing the color change of LFSA control and test line.
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