BBE02 disruption mutants of Borrelia burgdorferi B31 have a highly transformable, infectious phenotype
BBE02 disruption mutants of Borrelia burgdorferi B31 have a highly transformable, infectious phenotype
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DOI：
10.1128/iai.72.12.7147-7154.2004
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发表时间：
2004-12-01
影响因子：
3.1
通讯作者：
Watanabe, H
中科院分区：
文献类型：
--
作者：
Kawabata, H;Norris, SJ;Watanabe, H
我们通过灭活BBE02（线性质粒lp25上的一个假定的限制性修饰基因）构建了高度可转化和感染性的伯氏疏螺旋体B31。本研究使用低传代数B31克隆5A4（包含所有质粒）和5A18（lp28-4（-）lp56（-）），并通过同源重组破坏BBE02。穿梭载体 pBSV2C03::gntDeltakan 的转化效率从
We constructed highly transformable and infectious Borrelia burgdorferi B31 by inactivating BBE02, a putative restriction-modification gene on the linear plasmid lp25. The low-passage-number B31 clones 5A4 (containing all plasmids) and 5A18 (lp28-4(-) lp56(-)) were used for this study, and BBE02 was disrupted by homologous recombination. The transformation efficiency with the shuttle vector pBSV2C03::gntDeltakan was increased from