Marine diatoms grown in chemostats under silicate or ammonium limitation. II. Transient response of Skeletonema costatum to a single addition of the limiting nutrient
Marine diatoms grown in chemostats under silicate or ammonium limitation. II. Transient response of Skeletonema costatum to a single addition of the limiting nutrient
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DOI：
10.1007/bf00390940
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发表时间：
1976-04
期刊：
影响因子：
2.4
通讯作者：
H. L. Conway;H. L. Conway;P. Harrison;C. Davis
中科院分区：
文献类型：
--
作者：
H. L. Conway;H. L. Conway;P. Harrison;C. Davis
中肋骨条藻在铵或硅酸盐限制的恒化器中以不同的稳态生长速率生长。通过单一添加限制性营养物铵或硅酸盐，使培养物从其稳态条件扰动。瞬态响应之后，通过测量营养消失的liliting扰动实验表明，可以区分三种不同的模式的吸收限制养分：浪涌吸收（Vs），内部控制的吸收（Vi），和外部控制的吸收（Ve）。这三种模式的吸收的解释，并讨论了它们之间的关系，限制养分的吸收控制。非限制性营养素的吸收速率被证明是抑制在限制性营养素的吸收激增。动力学摄取参数，KsandVmax，从外部控制的摄取段，Ve期间获得的数据。相同的Vmax值为0。12 h-1，得到硅酸盐或铵限制下。在NH_4-N_1 ~（-1）和Si_1 ~（-1）条件下，K_s分别为0.4 μ g ~（-1）和0.7 μ g ~（-1）。在氮限制培养物上进行的短期15 N摄取率测量似乎是V或Vi的组合，或在较低的底物浓度V和Ve。这是很难分开这些不同的摄取模式在批或示踪剂实验，并随后在解释的问题进行了讨论。
Skeletonema costatumwas grown at different steady-state growth rates in ammonium or silicate-limited chemostats. The culture was perturbed from its steady-state condition by a single addition of the limiting nutrients ammonium or silicate. The transient response was followed by measuring nutrient disappearance of the liliting perturbation experiment indicate that three distinct modes of uptake of the limiting nutrient can be distinguished; surge uptake (Vs), internally controlled uptake (Vi), and externally controlled uptake (Ve). An interpretation of these three modes of uptake is given and their relation to control of uptake of the limiting nutrient is discussed. The uptake rates of the non-limiting nutrients were shown to be depressed during the surge of the uptake of the limiting nutrient. Kinetic uptake parameters,KsandVmax, were obtained from data acquired during the externally controlled uptake segment,Ve. The sameVmaxvalue of 0. 12 h-1, was obtained under either silicate or ammonium limitation. Estimates ofKswere 0.4 μg-at NH4-N l-1and 0.7 μg-at Si l-1. Short-term15N uptake-rate measurements conducted on nitrogen-limited cultures appear to be a combination ofVsorVi, or at lower substrate concentrationsVsandVe. It is difficult to separate these different uptake modes in batch or tracer experiments, and ensuing problems in interpretation are discussed.