Available organic nitrogen in temperate, subtropical, and tropical soils extracted with different solutions
Available organic nitrogen in temperate, subtropical, and tropical soils extracted with different solutions
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DOI：
10.1007/s00374-007-0240-8
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发表时间：
2008-02
影响因子：
6.5
通讯作者：
Miwa Okamoto;K. Okada
中科院分区：
文献类型：
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作者：
Miwa Okamoto;K. Okada
通过化学溶液提取有机氮已用于评估土壤中有效氮的含量。我们测试了几种解决方案从热带、亚热带和温带土壤中提取有机氮的效率。传统的 0.067 M 磷酸盐缓冲液成功地从所有检查的 23 种土壤中提取了有机氮。高性能尺寸排阻色谱图显示，无论土壤类型如何，所有磷酸盐缓冲液提取物均在约 7,800 Da 处出现单峰。峰面积与提取物的有机氮浓度相关。根据磷酸盐缓冲液的常规连续提取，热带土壤的有机氮保留率低于其他土壤。硫酸提取的有机氮与提取的铁量显着相关（P<0.001），表明铁可能在土壤中保留有机氮中发挥作用。
Extraction of organic N by chemical solutions has been used to assess the amount of available N in soil. We tested the efficiency of several solutions in extracting organic N from tropical, subtropical and temperate soils. A conventional 0.067 M phosphate buffer successfully extracted organic N from all 23 soils examined. High-performance size exclusion chromatograms showed a single peak at about 7,800 Da for all phosphate buffer extracts irrespective of soil types. The peak area correlated with the organic N concentration of extracts. Tropical soils had lower retention of organic N than other soils according to the conventional and sequential extraction with phosphate buffer. Organic N extracted with sulfuric acid was significantly (P< 0.001) correlated with the amount of extracted Fe, suggesting that Fe might play a role in the retention of organic N in soil.