Rabbit calcium-sensing receptor (CASR) gene: chromosome location and evidence for related genes.
Rabbit calcium-sensing receptor (CASR) gene: chromosome location and evidence for related genes.
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兔钙敏感受体（CASR）基因：染色体位置和相关基因的证据。
DOI：
10.1159/000015354
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Hendy,GN
中科院分区：
文献类型：
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作者：
Martin-DeLeon,PA;Canaff,L;Korstanje,R;Bhide,V;Selkirk,M;Hendy,GN
CaSR基因编码的钙敏感受体调节细胞的多种功能，在钙稳态中起着重要作用。在这里，我们提供了兔CaSR基因外显子VII的序列，表明它在核苷酸水平上与人类基因有91%的同源性，在氨基酸水平上有95%的同源性。使用从基因组文库中分离的粘粒，通过荧光原位杂交将该基因定位到染色体14q11上，并通过流式分选染色体的PCR扩增独立地证实了这一点。此外，粘粒还在其他四条染色体上检测到频率较低的位置：3q、5p、8p和13p。其中两个位点(5p和13p)也被一个相关但不相同的粘粒检测到，并映射到与小鼠钙敏感受体相关序列(CaSR-Rs)同源的区域；这表明它们可能代表兔的CaSR相关序列。这些数据支持在G蛋白偶联受体(GPCR超家族)中存在与钙敏感受体相关的基因家族，并扩展了现有的人和兔染色体的同源性知识。
Diverse cellular functions are regulated by the calcium-sensing receptor, encoded by the CASR gene, which plays an important role in calcium homeostasis. Here we provide the sequence for exon VII of the rabbit CASR gene and show that it is 91% identical to the human gene at the nucleotide level, and 95% identical at the amino acid level. The gene was mapped by fluorescence in situ hybridization, using a cosmid isolated from a genomic library, to chromosome 14q11 and this was confirmed independently by PCR amplification of flow sorted chromosomes. In addition, the cosmid detected sites with lower frequencies on four other chromosomes: 3q, 5p, 8p, and 13p. Two of these sites (5p and 13p) were also detected by a related but unidentical cosmid, and map to regions that are homologous to the mouse calcium-sensing receptor related sequences (Casr-rs); suggesting that they may represent CASR-related sequences in the rabbit. The data support the presence of a family of genes related to the calcium-sensing receptor in the G-protein coupled receptor (GPCR) superfamily, as well as extend the existing knowledge of homology for several human and rabbit chromosomes.