The effects of pore size in bilayered poly(lactide-co-glycolide) scaffolds on restoring osteochondral defects in rabbits
The effects of pore size in bilayered poly(lactide-co-glycolide) scaffolds on restoring osteochondral defects in rabbits
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双层聚丙交酯-乙交酯共聚物支架孔径对兔骨软骨缺损修复的影响
DOI：
10.1002/jbm.a.34683
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发表时间：
2014-01-01
影响因子：
4.9
通讯作者：
Ding, Jiandong
中科院分区：
文献类型：
--
作者：
Duan, Pingguo;Pan, Zhen;Ding, Jiandong
双层多孔支架由于其在修复骨软骨缺损方面的巨大潜力而引起了人们的兴趣。然而，确定双层多孔支架的最佳孔径仍然是一个重要的问题。本研究使用兔骨软骨缺损模型研究了双层支架中孔径的体内效应。我们制作了五种类型的集成双层聚（丙交酯-共-乙交酯）（PLGA）支架，在软骨和骨层具有不同的孔径（50-100 μ m，100-200 μ m，200-300 μ m和300-450 μ m）。将接种或不接种同种异体骨髓间充质干细胞（BMSCs）的双层支架亚组植入兔骨软骨缺损中。所有的细胞/支架复合结构都支持关节软骨和软骨下骨的同时再生，但是在软骨层中具有100-200 μ m孔和骨层中具有300-450 μ m孔的细胞接种的PLGA支架中观察到最好的结果。我们的研究支持了这样一个概念，即在组织工程支架设计中应考虑孔径对骨软骨修复的影响。(c)2013 Wiley Periodicals，Inc. J Biomed Mater Res Part A：102A：180-192，2014.
Bilayered porous scaffolds have recently attracted interest because of their considerable promise for repairing osteochondral defects. However, determination of optimal pore size in bilayered porous scaffolds remains an important issue. This study investigated the in vivo effects of pore size in bilayered scaffolds using a rabbit model of osteochondral defects. We fabricated five types of integrated bilayered poly(lactide-co-glycolide) (PLGA) scaffolds with different pore sizes in the chondral and osseous layers (50-100 mu m, 100-200 mu m, 200-300 mu m, and 300-450 mu m). A subset of bilayered scaffolds seeded with or without allogenic bone marrow mesenchymal stem cells (BMSCs) was implanted in rabbit osteochondral defects. All of the cell/scaffold composite constructs supported the simultaneous regeneration of articular cartilage and subchondral bone, but the best results were observed in cell-seeded PLGA scaffolds with 100-200 mu m pores in the chondral layer and 300-450 mu m pores in the osseous layer. Our study supports the concept that the effects of pore size on osteochondral repair should be taken into consideration during scaffold design for tissue engineering. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 180-192, 2014.