Substratum nanotopography and the adhesion of biological cells. Are symmetry or regularity of nanotopography important?
Substratum nanotopography and the adhesion of biological cells. Are symmetry or regularity of nanotopography important?
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DOI：
10.1016/s0301-4622(01)00247-2
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发表时间：
2001-12-25
影响因子：
3.8
通讯作者：
Wilkinson, CDW
中科院分区：
文献类型：
--
作者：
Curtis, ASG;Casey, B;Wilkinson, CDW
动物细胞生活在这样的环境中，它们周围的许多特征都是纳米级的，例如胶原分子的细节。细胞会对这种大小的物体做出反应吗？如果是的话，它们对分子的哪些特征做出反应？在这里，我们展示了，通过在二氧化硅中制造纳米特征，通过在聚己内酯中浇铸反向特征，并在其上培养脊椎动物细胞，细胞对特征的粘附性做出反应。对于悬崖，悬崖边缘的附着力增强，而有序阵列中的凹坑或柱子减少附着力。这一结果暗示了细胞黏附中的有序拓扑结构和可能的对称性效应。在羧酸盐表面2微米直径的颗粒与这些表面的粘附性方面也得到了类似的结果。这些结果与非生物纳米系统最近的预测是一致的。(C)2001 Elsevier Science B.V.保留所有权利。
Animal cells live in environments where many of the features that surround them are on the nanoscale, for example detail on collagen molecules. Do cells react to objects of this size and if so, what features of the molecules are they responding to? Here we show, by fabricating nanometric features in silica and by casting reverse features in polycaprolactone and culturing vertebrate cells in culture upon them, that cells react in their adhesion to the features. With cliffs, adhesion is enhanced at the cliff edge, while pits or pillars in ordered arrays diminish adhesion. The results implicate ordered topography and possibly symmetry effects in the adhesion of cells. Parallel results were obtained in the adhesion of carboxylate-surfaced 2-mum-diameter particles to these surfaces. These results are in agreement with recent predictions from non-biological nanometric systems. (C) 2001 Elsevier Science B.V. All rights reserved.