Micromonas pusilla Virus: the Virus Growth Cycle and Associated Physiological Events Within the Host Cells; Host Range Mutation
Micromonas pusilla Virus: the Virus Growth Cycle and Associated Physiological Events Within the Host Cells; Host Range Mutation
复制标题
小单胞菌病毒：宿主细胞内的病毒生长周期和相关生理事件；
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
A. T. Chan
中科院分区：
文献类型：
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作者：
R. Waters;A. T. Chan
总结
光合海洋鞭毛虫微小微单胞菌Micromonas pusilla（Butch.）Manton et Parke（Prasinophyceae）及其胞质病毒M. pusilla病毒（MPV）。将宿主细胞维持在液体培养物中。通过终点稀释进行抗性滴定，使用宿主叶绿素的损失作为感染性病毒存在的指示剂。病毒生长周期的特征是蚀食期为3h，潜伏期为7h，总裂解周期为14h。平均爆发大小为每个细胞72个感染性颗粒。CO_2光合作用的抑制开始接种病毒后2小时。几乎立即减少在体内叶绿素a荧光感染细胞是光依赖性的。M. pusilla细胞可以在细胞表面突变为病毒抗性。MPV宿主范围突变株在不同的M. pusilla。
Summary
The photosynthetic marine flagellate Micromonas pusilla (Butch.) Manton et Parke (Prasinophyceae) and its cytoplasmic virus, M. pusilla virus (MPV), were cloned. Host cells were maintained in liquid culture. Infectivity titration was by endpoint dilution, using loss of host chlorophyll as an indicator of the presence of infective virus. The virus growth cycle was characterized by an eclipse period of 3 h, a latent period of 7 h and a total lytic cycle of 14 h. The average burst size was 72 infective particles per cell. Inhibition of CO2 photoassimilation began 2 h after inoculation with virus. An almost immediate decrease in in vivo chlorophyll a fluorescence in infected cells was light-dependent. M. pusilla cells can mutate to virus resistance at the cell surface. Host range mutants of MPV exhibited variable infectivity in different strains of M. pusilla.