A structure prediction for the ligand-binding region of the integrin beta subunit: Evidence for the presence of a von Willebrand factor A domain
A structure prediction for the ligand-binding region of the integrin beta subunit: Evidence for the presence of a von Willebrand factor A domain
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DOI：
10.1016/s0014-5793(96)01368-3
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发表时间：
1997-01-06
期刊：
影响因子：
3.5
通讯作者：
Humphries, MJ
中科院分区：
文献类型：
--
作者：
Tuckwell, DS;Humphries, MJ
整合素是细胞表面受体的一个家族，介导生物学上重要的粘附相互作用。整合素-配体结合已被广泛研究，因为它具有开发抗粘附疗法的潜力，但这种相互作用的分子基础仍然知之甚少。β亚基n端附近的一个保守区域似乎在配体结合中特别重要，但迄今为止，该区域尚未被孤立表达。作为表达和潜在结构确定的前奏，我们对该区域进行了详细的结构预测。一级、二级和三级结构分析表明，该区域折叠成血管性血液病因子a域，从而可能将先前表征的模块放置在关键功能区的中心。
The integrins are a family of cell surface receptors that mediate biologically important adhesive interactions. Integrin-ligand binding has been extensively studied because of the potential for the development of anti-adhesive therapies, but the molecular basis of this interaction is still poorly understood. A conserved region near the N-terminus of the beta subunit appears to be of particular importance in ligand binding, but to date this domain has not been expressed in isolation. As a prelude to expression and potential structure determination, we have performed a detailed structure prediction for this region. Primary, secondary and tertiary structure analyses indicate that the region folds into a von Willebrand factor A-domain, thereby potentially placing a previously characterised module at the centre of a key functional region.