Emerging investigator series: quantification of multiwall carbon nanotubes in plant tissues with spectroscopic analysis
Emerging investigator series: quantification of multiwall carbon nanotubes in plant tissues with spectroscopic analysis
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新兴研究者系列：利用光谱分析定量植物组织中的多壁碳纳米管
DOI：
10.1039/c8en01252k
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Yang, Yu
中科院分区：
文献类型：
--
作者：
Das, Kamol K.;Nava, Valeria;Chang, Che-Wei;Chan, James W.;Xing, Baoshan;Yang, Yu
如果农业植物接触到碳纳米管(CNTs)，它们可能会从生长介质中吸收碳纳米管，并将它们转移到不同的组织中。此外，碳纳米管在农业上的应用近年来引起了越来越多的关注，因为它们可以促进种子萌发，提高作物产量，并显示出其他好处。为了评价碳纳米管的环境效应，优化其在农业上的应用，对植物组织中碳纳米管的定量是必不可少的。本研究采用硝酸和硫酸连续消化法从植物组织中提取了纯净的(p-)和羧基功能化的(c-)多壁碳纳米管(MWCNTs)。提取的多壁碳纳米管用非离子表面活性剂Triton X-100稳定，并用紫外-可见(UV-Vis)光谱分析测定植物(生菜)组织中多壁碳纳米管的浓度。MWCNT的浓度与800 nm处的吸光度呈线性关系。叶、茎、根组织中p-和c-多壁碳纳米管的检出限分别为0.10-0.12、0.070-0.081、0.019-0.18μg mg−-1。将该方法应用于生菜(Lactuca sativa，cv.)黑籽Simpson)在培养液中分别加入5、10、20 mg L−1的p-多壁碳纳米管和c-多壁碳纳米管。生菜根中检测到0.2 1±0.0 5~4.5 7±0.39μg mg−1 p-μ碳纳米管和0.2 0±0.17~0.75±0.2 5 mg−1 c-MWCNTs，与溶液中碳纳米管剂量呈正相关。我们利用一种可广泛使用的技术开发了一种快速定量植物组织中碳纳米管的方法，该方法可以可靠地分析植物组织中的碳纳米管，并为评估碳纳米管的环境影响和管理碳纳米管的农业应用提供关键信息。
If agricultural plants are exposed to carbon nanotubes (CNTs), they can potentially take up the CNTs from growth media and translocate them to their different tissues. In addition, agricultural application of CNTs recently attracted increasing attention, as they could promote germination, enhance crop yield, and exhibit other benefits. For evaluating the environmental effects of CNTs and optimizing their agricultural application, it is essential to quantify CNTs in plant tissues. In this study, pristine (p-) and carboxyl-functionalized (c-) multiwall CNTs (MWCNTs) were extracted from plant tissues by a sequential digestion with nitric acid (HNO3) and sulfuric acid (H2SO4). The extracted MWCNTs were stabilized with nonionic surfactant Triton X-100 and analyzed with ultraviolet-visible (UV-vis) spectroscopic analysis to measure the concentration of the MWCNTs in plant (lettuce) tissues. The MWCNT concentration was linearly correlated with the absorbance at 800 nm. The detection limit for p- and c-MWCNTs was achieved at 0.10–0.12, 0.070–0.081, 0.019–0.18 μg mg−1 for leaf, stem, and root tissues, respectively. The developed method was applied for lettuce (Lactuca sativa, cv. black seeded Simpson) hydroponically grown with 5, 10, 20 mg L−1 of p-MWCNTs and c-MWCNTs in the culture solution. We detected 0.21 ± 0.05–4.57 ± 0.39 μg mg−1 p-MWCNTs and 0.20 ± 0.17–0.75 ± 0.25 μg mg−1 c-MWCNTs in the lettuce roots, positively correlated with the dose of CNTs in solution. We have developed a method for rapid quantification of CNTs in plant tissues using a widely-accessible technique, which can enable reliable analysis of CNTs in plant tissues and provide critical information for evaluating the environmental implications and managing agricultural application of CNTs.
影响因子：
6.2
作者：
Hambardzumyan, Arayik;Foulon, Laurence;Aguie-Beghin, Veronique
通讯作者：
Aguie-Beghin, Veronique
用于复杂混合物中碳纳米管检测的热重-质谱法。
影响因子：
11.4
作者：
D. Plata;C. Reddy;P. Gschwend
通讯作者：
P. Gschwend