Docosahexaenoic acid-enriched fish oil consumption modulates immunoglobulin responses to and clearance of enteric reovirus infection in mice
Docosahexaenoic acid-enriched fish oil consumption modulates immunoglobulin responses to and clearance of enteric reovirus infection in mice
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DOI：
10.1093/jn/138.4.813
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发表时间：
2008-04-01
影响因子：
4.2
通讯作者：
Pestka, James J.
中科院分区：
文献类型：
--
作者：
Beli, Eleni;Li, Maoxiang;Pestka, James J.
我们假设，消耗（n-3）PUFA，二十二碳六烯酸（DHA），调节粘膜免疫反应，肠道感染呼吸道肠道孤儿病毒（呼肠孤病毒），肠道病原体的模型。给小鼠喂食含有10 g/kg玉米油和60 g/kg高油酸红花油的AIN-93 G对照饮食，或含有10 g/kg玉米油和60 g/kg富含DHA的鱼油的AIN-93 G，持续4周，然后用呼肠孤病毒株1型Lang（T1/L）口服管饲。呼肠孤病毒特异性伊加抗体是第一次检测到的小鼠粪便中喂养的控制饮食在感染后6天（PI），并在8和10天PI进一步升高。免疫后6、8d，DHA组小鼠的伊加应答相似，但10 d时IgA免疫应答显著高于对照组（P < 0.05）。呼肠孤病毒特异性血清伊加和IgG（2a）在喂食对照或DHA饮食的小鼠中均被诱导。来自对照组和DHA组的离体派伊尔斑、固有层和脾培养物的呼肠孤病毒特异性伊加和IgG（2a）分泌是相当的。尽管两组小鼠每肠携带相似数量的呼肠孤病毒空斑形成单位，但在感染后2、4和6天，饲喂DHA的小鼠在粪便中排出的病毒RNA几乎是对照小鼠的10倍（P < 0.05）。然而，在8和10 d时，两组中均未检测到病毒RNA。总之，这些数据表明，DHA的消费并没有显着改变粘膜或全身IG呼肠孤病毒的反应，但延迟清除肠道病毒。J.营养138：813-819，2008.
We hypothesized that consumption of the (n-3) PUFA, docosahexaenoic acid (DHA), modulates the mucosal immune response to enteric infection with respiratory enteric orphan virus (reovirus), a model intestinal pathogen. Mice were fed either AIN-93G control diet, containing 10 g/kg corn oil and 60 g/kg high oleic acid safflower oil, or AIN-93G, containing 10 g/kg corn oil and 60 g/kg DHA-enriched fish oil, for 4 wk and then orally gavaged with reovirus strain Type 1 Lang, (T1/L). Reovirus-specific IgA antibody was first detectable in the feces of mice fed a control diet at 6 d postinfection (PI) and was further elevated at 8 and 10 d PI. IgA responses in DHA-fed mice were similar at 6 and 8 d PI but greater at 10 d PI (P < 0.05). Both reovirus-specific serum IgA and IgG(2a) were comparably induced in mice fed control or DHA diets. Reovirus-specific IgA and IgG(2a) secretion by ex vivo Payer's patch, lamina propria, and spleen cultures derived from control and DHA groups were comparable. Although both groups carried similar numbers of reovirus plaque forming units per intestine, DHA-fed mice shed nearly 10 times more viral RNA in feces than control mice at 2, 4, and 6 d PI (P < 0.05). However, viral RNA was not detectable in either group at 8 and 10 d. Taken together, these data suggest that DHA consumption did not markedly alter mucosal or systemic Ig responses to reovirus but delayed clearance of the virus from the intestinal tract. J. Nutr. 138: 813-819, 2008.