Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells
Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells
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DOI：
10.1038/labinvest.2012.4
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发表时间：
2012-04-01
影响因子：
5
通讯作者：
Morii, Eiichi
中科院分区：
文献类型：
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作者：
Ikeda, Jun-ichiro;Mamat, Suhana;Morii, Eiichi
具有致癌潜力的肿瘤细胞可能仅限于一小部分称为癌症起始细胞(CICs)的细胞。CICS在体外高效形成集落，产生CIC和非CIC群体，维持低水平的活性氧(ROS)，表现出高的乙醛脱氢酶(ALDH)活性，并且大多处于细胞周期的静止状态。霍奇金淋巴瘤(HL)的CICs体积小，ROS水平低。在HL细胞系中检测CICs中ROS水平与ALDH活性的关系。ROS低和ALDH高的群体在半固体培养中形成菌落的效率比ROS高和ALDH低的群体更有效。ALDH高群体既产生ALDH低群体，又产生ALDH高群体，而ALDH低群体很少产生ALDH高群体。处于静止状态的细胞数在ROS-Low组显著多于ROS-HIGH组，而ALDH-HIGH组和ALDH-Low组无显著差异。这些发现表明，ALDH高和ROS低的细胞具有类似CIC的潜能，但它们的细胞周期状态不同，提示CICs由具有异质性特征的细胞组成。实验室调查(2012年)92,606-614；doi：10.1038/Labinvest.2012.4；2012年2月20日在线发布
Tumor cells with tumorigenic potential might be limited to a small population of cells, called cancer-initiating cells (CICs). CICs efficiently form colonies in vitro, yield both CIC and non-CIC populations, maintain reactive oxygen species (ROS) at low levels, show high aldehyde dehydrogenase (ALDH) activity, and are mostly in a quiescent state of the cell cycle. CICs of Hodgkin lymphoma (HL) are small in size, with low levels of ROS. The relationship between ROS level and ALDH activity in CICs was examined in HL cell lines. ROS-low and ALDH-high populations formed colonies in semi-solid cultures more efficiently than ROS-high and ALDH-low populations. ALDH-high populations yielded both ALDH-low and -high populations, whereas ALDH-low populations rarely yielded an ALDH-high population. The number of cells in a quiescent state was significantly greater in ROS-low than in ROS-high cells, whereas that of ALDH-high and ALDH-low cells was comparable to each other. These findings show that ALDH-high and ROS-low cells share CIC-like potential, but they differ in their cell cycle status, suggesting that CICs are comprised of cells with heterogeneous characteristics. Laboratory Investigation (2012) 92, 606-614; doi:10.1038/labinvest.2012.4; published online 20 February 2012