The immunomodulatory actions of prostaglandin E2 on allergic airway responses in the rat
The immunomodulatory actions of prostaglandin E2 on allergic airway responses in the rat
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DOI：
10.4049/jimmunol.169.7.3963
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发表时间：
2002-10-01
影响因子：
4.4
通讯作者：
Powell, WS
中科院分区：
文献类型：
--
作者：
Martin, JG;Suzuki, M;Powell, WS
据报道，前列腺素E(2)可抑制致敏受试者中变应原诱导的呼吸道反应。本研究旨在探讨前列腺素E(2)在过敏性哮喘动物模型中的抗炎作用机制。以百日咳杆菌为佐剂，用卵清蛋白致敏BN大鼠。一周后，雾化吸入卵清蛋白。一周后，动物用乌拉坦麻醉，插管，测量肺阻力(R-L)，持续8小时。在OVA激发前30min气管内分别注入PGE(2)(1杯和3杯生理盐水)。PGE(2)(3mug)可抑制早期反应。PGE(2)(1和3µg)均能抑制晚期反应。OVA激发的大鼠肺泡灌洗液可见嗜酸性粒细胞增多，表达IL-4和IL-5mRNA的细胞数量增加。这些反应可被PGE(2)抑制。支气管肺泡灌洗液中半胱氨酰-白三烯水平在OVA激发后升高，在PGE(2)后下降至与假刺激动物相当的水平。我们得出结论，PGE(2)是一种有效的抗炎药，可能通过减少变应原诱导的Th2细胞激活和半胱氨基白三烯的合成而发挥作用。
PGE(2) has been reported to inhibit allergen-induced airway responses in sensitized human subjects. The aim of this study was to investigate the mechanism of anti-inflammatory actions of PGE(2) in an animal model of allergic asthma. BN rats were sensitized to OVA using Bordetella pertussis as an adjuvant. One week later, an aerosol of OVA was administered. After a further week, animals were anesthetized with urethan, intubated, and subjected to measurements of pulmonary resistance (R-L) for a period of 8 h after OVA challenge. PGE(2) (1 and 3 mug in 100 mul of saline) was administered by insufflation intratracheally 30 min before OVA challenge. The early response was inhibited by PGE(2) (3 mug). The late response was inhibited by both PGE(2) (1 and 3 mug). Bronchoalveolar lavage fluid from OVA-challenged rats showed eosinophilia and an increase in the number of cells expressing IL-4 and IL-5 mRNA. These responses were inhibited by PGE(2). Bronchoalveolar lavage fluid levels of cysteinyl-leukotrienes were elevated after OVA challenge and were reduced after PGE(2) to levels comparable with those of sham challenged animals. We conclude that PGE(2) is a potent anti-inflammatory agent that may act by reducing allergen-induced Th2 cell activation and cysteinyl-leukotriene synthesis in the rat.