Habitual exercise induced resistance to oxidative stress
Habitual exercise induced resistance to oxidative stress
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DOI：
10.1080/10715760500183300
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发表时间：
2005-09-01
影响因子：
3.3
通讯作者：
Takeuchi, T
中科院分区：
文献类型：
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作者：
Nakatani, K;Komatsu, M;Takeuchi, T
我们研究了习惯性运动(HE)是否调节了肾癌致癌物Fe-NTA(Fe-NTA)诱导的DNA氧化损伤水平和对氧化应激的反应性。在为期10周的方案中，两组大鼠要么保持久坐不动，要么每周5天每天游泳15-60分钟，无论是否有相当于其体重5%的体重。然后注射Fe-NTA，注射后1h处死大鼠。测定膈、肾超氧化物歧化酶(SOD)活性，测定肾脏8-羟基脱氧鸟苷(80HdG)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)水平，测定肾脏OGG1蛋白水平。HE大鼠膈、肾组织中超氧化物歧化酶活性明显升高。HE本身对80HdG水平无影响，但对Fe-NTA诱导的80HdG有明显的抑制作用，且抑制程度与运动强度有关。这些结果表明，HE诱导了对氧化应激的抵抗，至少在起始阶段，抑制了癌症的发生。
We investigated whether habitual exercise (HE) modulates levels of oxidative DNA damage and responsiveness to oxidative stress induced by renal carcinogen Fe-nitrilotriacetic acid (Fe-NTA). During a ten week protocol, two groups of rats either remained sedentary or underwent swimming for 15 - 60 min per day, 5 days per week, with or without a weight equivalent to 5% of their body weight. Then we injected Fe-NTA and sacrificed the rats I h after the injection. We determined the activity of superoxide dismutase (SOD) in diaphragm and kidney, evaluated levels of 8-hydroxydeoxyguanosine (80HdG), catalase, and glutathione peroxidase, and assayed OGG1 protein levels in kidney. SOD activity in the diaphragm and kidney was increased in HE rats. By itself, HE had no effect on the level of 80HdG, but it did significantly suppress induction of 80HdG by Fe-NTA, and the amount of suppression correlated with intensity of exercise. These results suggest that HE induces resistance to oxidative stress and, at least at the initiation stage, inhibits carcinogenesis.