Beneficial Effects of Tocotrienol and Tocopherol on Bone Histomorphometric Parameters in Sprague-Dawley Male Rats After Nicotine Cessation
Beneficial Effects of Tocotrienol and Tocopherol on Bone Histomorphometric Parameters in Sprague-Dawley Male Rats After Nicotine Cessation
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DOI：
10.1007/s00223-008-9190-x
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发表时间：
2009-01-01
影响因子：
4.2
通讯作者：
Norazlina, Mohamed
中科院分区：
文献类型：
--
作者：
Hermizi, Hapidin;Faizah, Othman;Norazlina, Mohamed
本研究旨在确定尼古丁治疗后三种形式的维生素E补充剂对成年雄性大鼠模型骨组织形态学参数的影响。将大鼠分为7组：基线组（B，未处理处死）、对照组（C，生理盐水4个月）、尼古丁组（N，尼古丁2个月）、尼古丁戒烟组（NC）、生育三烯醇增强组分（TEF）、γ -生育三烯醇（GTT）和α -生育酚（ATF）。NC组、TEF组、GTT组和ATF组分两期治疗。前2个月给予尼古丁（7 mg/kg），随后2个月停止给药，并开始服用相应的维生素E制剂（60 mg/kg）， NC组除外，允许其在不治疗的情况下恢复。与C组相比，N和NC组大鼠的骨小梁体积、矿物附着率（MAR）和骨形成率（BFR/BS）均较低，单标记表面和破骨细胞表面均较高。维生素E治疗逆转了尼古丁的这些作用。TEF组和GTT组的骨小梁厚度显著高于C组，但侵蚀面（ES/BS）显著低于ATF组。生育三烯醇处理组ES/BS低于ATF组。GTT组MAR和BFR/BS显著高于TEF和ATF组。综上所述，尼古丁会导致严重的骨质流失，而维生素E补充剂不仅会逆转这种影响，还会显著刺激骨骼形成，高于基线值。与生育酚相比，生育三烯醇表现出稍好的效果。因此，维生素E，特别是GTT，可能具有修复慢性吸烟引起的骨损伤的治疗潜力。
This study was conducted to determine the effectiveness of three forms of vitamin E supplements following nicotine treatment on bone histomorphometric parameters in an adult male rat model. Rats were divided into seven groups: baseline (B, killed without treatment), control (C, normal saline for 4 months), nicotine (N, nicotine for 2 months), nicotine cessation (NC), tocotrienol-enhanced fraction (TEF), gamma-tocotrienol (GTT), and alpha-tocopherol (ATF). Treatments for the NC, TEF, GTT, and ATF groups were performed in two phases. For the first 2 months they were given nicotine (7 mg/kg), and for the following 2 months nicotine administration was stopped and treatments with respective vitamin E preparations (60 mg/kg) were commenced except for the NC group, which was allowed to recover without treatment. Rats in the N and NC groups had lower trabecular bone volume, mineral appositional rate (MAR), and bone formation rate (BFR/BS) and higher single labeled surface and osteoclast surface compared to the C group. Vitamin E treatment reversed these nicotine effects. Both the TEF and GTT groups, but not the ATF group, had a significantly higher trabecular thickness but lower eroded surface (ES/BS) than the C group. The tocotrienol-treated groups had lower ES/BS than the ATF group. The GTT group showed a significantly higher MAR and BFR/BS than the TEF and ATF groups. In conclusion, nicotine induced significant bone loss, while vitamin E supplements not only reversed the effects but also stimulated bone formation significantly above baseline values. Tocotrienol was shown to be slightly superior compared to tocopherol. Thus, vitamin E, especially GTT, may have therapeutic potential to repair bone damage caused by chronic smoking.