Applying vibration in early postmenopausal osteoporosis promotes osteogenic differentiation of bone marrow-derived mesenchymal stem cells and suppresses postmenopausal osteoporosis progression
Applying vibration in early postmenopausal osteoporosis promotes osteogenic differentiation of bone marrow-derived mesenchymal stem cells and suppresses postmenopausal osteoporosis progression
复制标题
在绝经后骨质疏松症早期应用振动可促进骨髓间充质干细胞的成骨分化并抑制绝经后骨质疏松症的进展
DOI：
10.1042/bsr20191011
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发表时间：
2019-09-03
影响因子：
4
通讯作者：
Li, Liang
中科院分区：
文献类型：
--
作者：
Li, Huiming;Wu, Wenchao;Li, Liang
我们的目的是评估在绝经后早期骨质疏松症（PMO）中应用低震级振动（LMV）是否能抑制其进展，并探讨其潜在机制。将大鼠随机分为Sham（假手术）、Sham+V、OVX（去卵巢）、OVX+E2（苯甲酸雌二醇）、OVX+V（术后12-20周LMV）、OVX+Vi（术后1-20周LMV）组。LMV应用20分钟，每日1次，每周5天。V大鼠于术后12-20周转染LMV。6只大鼠于术后1 ~ 20周转染LMV。术后12 ~ 20周肌肉注射雌二醇（E2），每日1次，连续3天。分析胫骨的骨矿物质密度、生物力学性能和组织形态学参数。在体外，将大鼠骨髓间充质干细胞（rBMSCs）置于LMV中，每天30分钟，持续5天，或17β-E2加或不加1天的雌激素受体（ER）抑制剂ICI 182780 （ICI）预处理。进行mRNA和蛋白的表达。数据显示，LMV能提高大鼠的骨密度、骨强度和骨量，且Vi的作用强于E2。在体外，与E2相比，LMV上调Runx2、Osx、Col I和OCN mRNA和蛋白的表达，下调PPARγ的表达。LMV和E2对rBMSCs的作用均被ICI抑制。总之，早期PMO中的LMV抑制其进展，这与rBMSCs的成骨分化有关，通过上调ERα和激活典型Wnt通路。因此，LMV在抑制PMO进展方面可能优于E2。
We aimed to evaluate whether applying low magnitude vibration (LMV) in early postmenopausal osteoporosis (PMO) suppresses its progression, and to investigate underlying mechanisms. Rats were randomly divided into Sham (Sham-operated), Sham+V, OVX (ovariectomized), OVX+E2 (estradiol benzoate), OVX+V (LMV at 12–20 weeks postoperatively), and OVX+Vi (LMV at 1–20 weeks postoperatively) groups. LMV was applied for 20 min once daily for 5 days weekly. V rats were loaded with LMV at 12–20 weeks postoperatively. Vi rats were loaded with LMV at 1–20 weeks postoperatively. Estradiol (E2) rats were intramuscularly injected at 12–20 weeks postoperatively once daily for 3 days. The bone mineral densities (BMDs), biomechanical properties, and histomorphological parameters of tibiae were analyzed. In vitro, rat bone marrow-derived mesenchymal stem cells (rBMSCs) were subjected to LMV for 30 min daily for 5 days, or 17β-E2 with or without 1-day pretreatment of estrogen receptor (ER) inhibitor ICI 182,780 (ICI). The mRNA and protein expresion were performed. Data showed that LMV increased BMD, bone strength, and bone mass of rats, and the effects of Vi were stronger than those of E2. In vitro, LMV up-regulated the mRNA and protein expressions of Runx2, Osx, Col I, and OCN and down-regulated PPARγ, compared with E2. The effects of both LMV and E2 on rBMSCs were inhibited by ICI. Altogether, LMV in early PMO suppresses its progression, which is associated with osteogenic differentiation of rBMSCs via up-regulation of ERα and activation of the canonical Wnt pathway. LMV may therefore be superior to E2 for the suppression of PMO progression.