Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate
Effect of Oxygen Tension and Medium Components on Monomer Distribution of Alginate
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氧张力和介质成分对海藻酸盐单体分布的影响
DOI：
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发表时间：
2015
影响因子：
3
通讯作者：
F. Sanin
中科院分区：
文献类型：
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作者：
Ç. Kıvılcımdan Moral;Ö. Doğan;F. Sanin
藻酸盐是由甘露糖醛酸和古洛糖醛酸单体组成的天然生物聚合物。它由藻类和一些固氮菌和假单胞菌属的物种产生。本研究旨在研究溶解氧张力（DOT）和生长培养基底物和钙浓度对棕色固氮菌ATCC® 9046在发酵罐中产生的藻酸盐单体组成的影响。结果表明，海藻酸盐产量随着DOT从1增加到5%而增加。当蔗糖浓度为20 g/L、钙浓度为50 mg/L、DOT浓度为5%时，海藻酸产量最高，为4.51 g/L。在这些条件下，藻酸盐富含甘露糖醛酸（高达61%），并且在低钙浓度下特别高。另一方面，在极端条件下，如高DOT水平（10%DOT）和低蔗糖浓度（10 g/L），古洛糖醛酸占主导地位（范围在65%和100%之间）。
Alginate is a natural biopolymer composed of mannuronic and guluronic acid monomers. It is produced by algae and some species of Azotobacter and Pseudomonas. This study aims to investigate the effect of dissolved oxygen tension (DOT) and growth medium substrate and calcium concentrations on the monomeric composition of alginate produced by Azotobacter vinelandii ATCC® 9046 in a fermenter. Results showed that alginate production increased with increasing DOT from 1 to 5 %. The highest alginate production was obtained as 4.51 g/L under 20 g/L of sucrose and 50 mg/L of calcium at 5 % DOT. At these conditions, alginate was rich in mannuronic acid (up to 61 %) and it was particularly high at low calcium concentration. On the other hand, at extreme conditions such as high DOT level (10 % DOT) and low sucrose concentration (10 g/L), guluronic acid was dominant (ranging between 65 and 100 %).