Low-Calorie Diet-Induced Reduction in Serum HDL Cholesterol Is Ameliorated in Obese Women with the-3826 G Allele in the Uncoupling Protein-1 Gene
Low-Calorie Diet-Induced Reduction in Serum HDL Cholesterol Is Ameliorated in Obese Women with the-3826 G Allele in the Uncoupling Protein-1 Gene
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DOI：
10.1620/tjem.219.337
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发表时间：
2009-12-01
影响因子：
2.2
通讯作者：
Sakane, Naoki
中科院分区：
文献类型：
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作者：
Hamada, Taku;Kotani, Kazuhiko;Sakane, Naoki
解偶联蛋白-1(UCP1)基因在调节体重和脂/脂蛋白代谢中起重要作用。我们的横断面研究表明，UCP-1基因-3826A/G多态的G/G基因型的受试者血清高密度脂蛋白胆固醇(HDLc)水平高于其他基因型的受试者。低水平的循环高密度脂蛋白胆固醇被认为是动脉粥样硬化的主要危险因素。因此，我们调查了在低卡路里饮食(LCD)干预期间，-3826A/G多态是否影响肥胖和血脂相关参数。对32名肥胖女性(年龄49.9+/-8.4岁)进行为期2个月的LCD治疗，测量治疗前后的人体测量、生理生化特征，限制每个受试者每天摄入相同的能量，如5120kJ/d。采用聚合酶链式反应-限制性片段长度多态法检测-3826A/G多态。A/A型6例，A/G型15例，G/G型11例。液晶显示器干预降低了所有受试者的体重(P<；0.001)和血清高密度脂蛋白胆固醇水平(P<；0.05)。G等位基因携带者和不携带G等位基因的受试者在体重、营养摄入量、能量消耗和脂肪氧化方面的变化水平没有差异。相反，在携带G等位基因的受试者中，高密度脂蛋白胆固醇水平的降低程度明显小于不携带G等位基因的受试者。提示UCP1基因-3826位G等位基因可能改善了肥胖女性LCD时血清高密度脂蛋白胆固醇水平的降低。
The uncoupling protein-1 (UCP1) gene is of major importance for regulation of body weight and lipid/lipoprotein metabolism. Our cross-sectional study has shown that subjects with the G/G genotype of the -3826 A/G polymorphism in the UCP-1 gene have higher levels of serum high-density lipoprotein cholesterol (HDL-C) than those with other genotypes. Low circulating HDL-C level has been regarded as a major atherosclerotic risk factor. We therefore investigated whether the -3826 A/G polymorphism affects the obesity- and lipid-related parameters during a low-calorie diet (LCD) intervention. In 32 obese women (49.9 +/- 8.4 years of age), anthropometric, physiological and biochemical characteristics were measured before and after a 2-month LCD treatment, which restricted each subject to the same energy intakes, such as 5,120 kJ/day. The -3826 A/G polymorphism was detected using a PCR-restriction fragment-length polymorphism method. There were 6 subjects with the A/A genotype, 15 with the A/G genotype and 11 with the G/G genotype. The LCD intervention decreased weight (P < 0.001) and serum HDL-C levels (P < 0.05) in all subjects. There was no difference in the levels of change in weight, nutrient intake, physiological measurements in energy expenditure, and fat oxidation between subjects with and without the G allele. In contrast, the degree of the reduction in the HDL-C levels was significantly smaller in subjects with the G allele than those without the G allele. These results suggest that the G allele at -3826 in the UCP1 gene may ameliorate the reduction in serum HDL-C levels in obese women during LCD.