Structure-function relationship of the extracellular calcium-sensing receptor
Structure-function relationship of the extracellular calcium-sensing receptor
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DOI：
10.1016/j.ceca.2003.10.018
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发表时间：
2004-03-01
期刊：
影响因子：
4
通讯作者：
Bai, M
中科院分区：
文献类型：
--
作者：
Bai, M
细胞外钙敏感受体(CAR)最初是从牛甲状旁腺克隆的，是一种G蛋白偶联受体。通过鉴定由CAR中的失活和激活突变引起的高钙和低钙紊乱，已经建立了克隆的CAR与感知和调节细胞外钙浓度的生理学相关性。克隆的CAR已在人胚胎肾细胞中稳定或瞬时表达，并在生理、生化和分子生物学方法阐明其调控和激活过程方面取得了重大进展。大量自然发生的CAR突变为旨在了解受体结构-功能关系的研究提供了宝贵的资源，包括CAR二聚化的功能重要性。反过来，这些自然发生的突变的特征阐明了涉及CAR异常的临床情况的发病机制，如家族性低钙尿性高钙血症和新生儿严重甲状旁腺功能亢进症。(C)2003爱思唯尔有限公司。保留所有权利。
The extracellular calcium-sensing receptor (CaR) originally cloned from bovine parathyroid gland is a G protein-coupled receptor. The physiological relevance of the cloned CaR for sensing and regulating the extracellular calcium concentration has been established by identifying hyper-and hypocalcemic disorders resulting from inactivating and activating mutations, respectively, in the CaR. The cloned CaR has been stably or transiently expressed in human embryonic kidney cells and significant progress has been made in elucidating its regulation and activation process using physiological, biochemical and molecular biological methods. A large collection of naturally occurring CaR mutations offers a valuable resource for studies aimed at understanding the structure-function relationships of the receptor, including functional importance of CaR dimerization. In turn, characterization of these naturally occurring mutations has clarified the pathogenesis of clinical conditions involving abnormalities in the CaR, such as familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism. (C) 2003 Elsevier Ltd. All rights reserved.